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Mouse Serine protease 23 (PRSS23) ELISA Kit

Mouse Serine protease 23 (PRSS23) ELISA Kit

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Specification

Product name Mouse Serine protease 23 (PRSS23) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine protease 23 (PRSS23) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS23 in samples. An antibody specific for PRSS23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS23 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS23 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRSS23; MGC5107; SIG13; SPUVE; ZSIG13; serine protease; umbilical endothelium

Product Properties

Kit components • Mouse Serine protease 23 microplate
• Mouse Serine protease 23 standard
• Mouse Serine protease 23 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine protease 23 (PRSS23) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRSS23. No significant cross-reactivity or interference between Mouse PRSS23 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PRSS23 encodes a member of the trypsin family of serine proteases. Mouse studies found a decrease of mRNA levels after ovulation was induced. This gene seems to be highly conserved in vertebrates and may be an important ovarian protease. PRSS23 is associated with cardiovascular diseases, hematological diseases, neurological diseases, cancer, endocrinological diseases, and urological diseases. The invention also features compounds which bind to and/or activate or inhibit the activity of PRSS23 as well as pharmaceutical compositions comprising such compounds. The invention also provides PRSS23 as a biomarker for diseases such as cardiovascular diseases, hematological diseases, neurological diseases, cancer, endocrinological diseases, and urological diseases.
Gene ID 76453
Alternative PRSS23; MGC5107; SIG13; SPUVE; ZSIG13; serine protease; umbilical endothelium
Accession Q9D6X6

Image & description

Fig.1. Mouse Serine protease 23 (PRSS23) Standard Curve.

Fig.1. Mouse Serine protease 23 (PRSS23) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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