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The most comprehensive cell status detection methods for cytology study

Date:2023-05-12 Views:554

Cytology experts around the world may encounter the following problems when doing cell status tests

What are the commonly used cell state detection indicators at present?

What are the methods for detecting cell proliferation?

What are the methods for detecting apoptosis?

How is DNA damage detected?

Take a look at Abbkine's answers......

 

Abbkine presents the products favored by cell research users, comprehensively covering the detection applications of cell proliferation, cytotoxicity, apoptosis, cell aging, oxidative damage and other aspects. The product types include the breakthrough apoptosis and cell proliferation kits with innovative ideas and unique advantages, as well as the detection kits with superior quality and stable performance. Meet your different experimental needs, help your cytology research career.

Cell proliferation assay

Cell proliferation results in many important changes, including DNA synthesis, increased cell metabolism, and proliferation-specific protein expression. Based on the next-generation AbFluor ™ fluorescence staining technology, Abbkine has developed products that accurately detect cell proliferation status directly from the DNA level, while also enabling high-throughput detection of cell proliferation status through the level of cell metabolism. The kit contains a patented proliferation-negative control, which is a perfect solution to the non-standardized proliferation test results. At present, it mainly includes EdU and CCK8 two commonly used detection methods.

Fig 1. Schematic diagram of CCK8 detection

Fig 2. Comparison of two detection methods, EdU and BrdU, EdU not only simplifies the steps, but also reduces the detection cost, and the detection results are higher signal-to-noise ratio.

 

Order Information

Product name Product NO. Size Price ($)
EdU Cell Proliferation Image Kit(Green Fluorescence)) KTA2030 100 T/500  T 119/189
EdU Cell Proliferation Image Kit (Orange Fluorescence) KTA2031 100 T/500  T 119/189
SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) BMU106-EN  

200 T/1000 T/10000 T

$29/69/529
Cell Counting Kit-8 (CCK-8) KTA1020 1000 T/10000 T 498/2998

 

1.Cell apoptosis detection

Apoptosis is a process of death in which cells respond to the physiological and pathological stimulation signals of the environment, changes in environmental conditions, or orderly changes in response to palliative damage. Apoptosis involves a series of morphological and molecular changes such as DNA breakage, cell membrane structure, mitochondrial membrane potential and protein expression. Abbkine has developed a series of kits for the multidimensional analysis of apoptosis.

 Category  Detection Method
Morphology  Microscopic observation
Cell Function Mitochondrial membrane potential detection、Annexin V/PI
Biochemical marker Apoptotic molecular marker detection, DNA damage detection

 

Fig 3. Annexin V-AbFluor™ 488 flow cytometry for apoptosis.

Fig 4.One-Step TUNEL Apoptosis Assay Kit detecting result of HeLa cells treated with DNase I for 24 h. The green fluorescence in this image is TUNEL stained positive cells.

Order Information

Product name Product NO. Size Price ($)
One-Step TUNEL Apoptosis Assay Kit(Green Fluorescence) KTA2010 50 T/100  T 259/459
One-step TUNEL Apoptosis Assay Kit(Orange Fluorescence) KTA2011 50 T/100  T 259/459
Annexin V-AbFluor™ 488/PI Apoptosis Detection kit KTA0002 50 T/100 T 109/179
Annexin V-EGFP/PI Apoptosis Detection kit KTA0005 20 T/50 T/100 T 49/99/169
Mitochondrial Membrane Potential Assay Kit (JC-1) KTA4001 20 T/100 T500 T 49/149/449
Caspase-1 Assay Kit (Colorimetric) KTA3020 20 T/50 T/100 T 89/159/289

 

  1. Detection of cytotoxic aging damage

In addition to the common indicators of proliferation and apoptosis, Abbkine also provides the detection products of cytotoxicity, aging, migration, invasion, DNA damage and other popular indicators, which are deeply loved by the majority of customers.

    Detection Method Example
Cell enzyme leakage lactate dehydrogenase (LDH)
Enzyme activity in mitochondria CCK8, MTT
Analyze living and dead cells Double staining of live/dead cells
Cell senescence SA-β-gal Detection
DNA damage Comet assay

Fig 5. SW480 cells induced by 1% FBS were stained by cell migration analysis kit (24-well plate, 8 µm)

 

Order Information

Product name Product NO. Size
LDH Cytotoxicity Assay Kit KTA1030 96 T/480 T
Senescence β-Galactosidase Staining Kit KTA3030 100 T
Live and Dead Cell Double Staining Kit KTA1001 100 T/500 T/2000 T
Cell Migration Assay Kit (24 well,8μM) KTA5010 12 T/12 T×4
Cell Invasion Assay Kit (24 well,8μM) KTA5011 12 T/12 T×4
Comet Assay Kit (3-Well Slides) KTA3040 15 T/75 T
Mitochondrial Permeability Transition Pore Assay KTA4002 50 T/100  T

 

The above cell state detection kits have the characteristics of complete reagents, easy operation and reliable experimental results, which meet the experimental needs of scientific researchers. Welcome "Abbkine fans" to pay more attention to Abbkine public number, there will be more scientific surprises waiting for you yo. At the same time, in order to provide readers with a platform to learn biological knowledge, Abbkine technical experts through unremitting efforts, will be a variety of high-end biological experiments of dry goods, later published in the wechat public account, please love science readers continue to pay attention to the Abbkine public account.

Abbkine focuses on proteomics and cytology and is committed to the innovation and development of antibodies, proteins, analytical reagents and kits to become a key enabler of life science research and development, drug discovery and other fields. We provide you with the most popular products for the users of protein and immunology research, from the basic immunology products, such as protein extraction quantification, to internal reference labeled antibodies, primary antibodies and secondary antibodies for immunology experiments. Cell research users' favorite products, from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining tracer and cell metabolism detection products, to cytokines and protein detection kits for cell culture, just to help your research career!