Beyond BrdU, New Generation Cell Proliferation Imaging Analysis Kit (EdU Method)
Common methods of cell proliferation mainly include MTT method, WST-1 method and CCK-8 method, which are mainly based on the activity of cells, thus reflecting the overall proliferation effect, but unable to detect individual proliferating cells. Recognized as the most accurate method to detect cell proliferation is to directly detect DNA synthesis in cells. Previously, BrdU method was the most commonly used method. However, the method requires DNA denaturation (such as acid denaturation, thermal denaturation or DNase digestion) to expose BrdU, thus binding to BrdU antibody. The whole experiment has many influencing factors and poor stability. Abbkine innovates and develops EdU method based on EdU incorporation and subsequent click reaction to solve this application difficulty: Product name Cat# Fluorescent characteristic…
Selection Strategy of Apoptosis Products- Late apoptosis
Apoptosis is an orderly and programmed death followed by cells under the influence of physiological or pathological factors in order to maintain internal environment stability. Apoptosis occurs in cells. First, the cell volume shrinks and the connection disappears. Then, the density of cytoplasm increases, mitochondrial membrane potential disappears, permeability changes, cytochrome C is released to cytoplasm, nuclear substance is concentrated, nuclear membrane nucleolus is broken, DNA is degraded into fragments, and finally apoptotic bodies are formed and engulfed by macrophages. Late apoptosis In cell apoptosis, especially in the late stage of apoptosis, chromosomal DNA will be broken, resulting in a large number of sticky 3'-OH ends. Under the action of deoxynucleotide terminal transferase (TdT), derivatives formed by deoxyuridine triphosphate nucleotide…
Selection Strategy of Apoptosis Products- Early apoptosis
Apoptosis is a dynamic process, which involves a series of complex biochemical reactions, expression regulation of multiple genes, signal transduction, cascade reactions involving multiple enzymes and multiple signal pathways. The cell feels the corresponding apoptosis signal stimulation, and a series of control switches in the cell are turned on or off, and activation of various enzymes triggers a series of cascade reactions. Different external factors initiate apoptosis in different ways, resulting in different signal transduction. Early apoptosis Feature 1: eversion of lipid membrane inside In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the lipid bilayer of the cell membrane. In the early stage of cell apoptosis, PS turns from the inner side of the lipid…
Complete solution of immunoprecipitation (IP)-break down the key steps of IP step by step!
Step 1: Understand what immunoprecipitation is? Why should I do immunoprecipitation? Immunoprecipitation (IP) is a method for purifying and enriching target proteins from specific mixtures (usually cell lysates or expression supernatants) by using antigen-antibody specific reactions. The traditional IP experiment is that after the antibody is combined with the target protein, it is incubated with agarose or magnetic beads coupled with Protein A /G (Fc fragment of binding antibody), and the bead-protein A/G- antibody-target protein complex is obtained by centrifugation. The complex is washed and eluted for subsequent downstream experiments. IP is an important step in many protein-related researches. It is used to study the existence, relative abundance, up-down regulation of protein expression, protein-to-stability and interaction of proteins. Summary: The…
CheKine™ Xanthine Oxidase Assay Kit, a great tool for study of hyperuricemia
Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans. Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. Most of the protein in the liver exists in a form with xanthine dehydrogenase activity, but it can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. In various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves…
CheKine™ Lactate Assay Kit, fast quantitative determination of L (+)-Lactate in various samples
Lactate (CH3CH(OH)COO-) is a metabolic compound formed in animals by the action of the enzyme lactate dehydrogenase. Lactate is produced in proliferating cells during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. D(-)-Lactate can also be found in blood but only at about 1-5% of the concentration of L(+)-Lactate. Lactic acid is the end product of glycolysis in the body, which can be produced when tissue hypoxia or pyruvic acid is not oxidized in time. Usually, the lactic acid obtained from metabolism does…
Want High accuracy and specificity? Yes, it’s Abbkine CheKine™ Catalase (CAT) Activity Assay Kit with great value
【Background】 Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from…
Observing three-dimensional dynamics of the nucleus in live cells, Abbkine will give you great experiences !
Live-cell imaging technique allows real-time examination of almost every aspect of cellular function under normal and experimental conditions, so it is especially popular with cytology researchers. Abbkine TraKine™ Pro is a series of cell-permeable organic fluorescent probes which can selectively recognize and label organelles. Currently, we launched the staining kits involve tubulin, lysosome, mitochondrion and nuclei. This paper introduces the TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution). Function of nucleus The nucleus is the largest and most important cell structure in eukaryotic cells, mainly composed of nuclear envelope, chromatin, nuclear skeleton, nucleolus and nucleosome. Nuclear membrane is a two-layer membrane that completely encloses the nucleus and separates the material within the membrane from the cytoplasm…
Caspase(1,2,3,4,6,8,9) Assay Kit- Simple and convenient, to boost your apoptosis study
【Tips】If you are studying whether a certain drug can trigger apoptosis which relys on the caspase related pathway, and want to detect the activity of caspase, this article is suitable for you! This article is especially fit for researchers who are working hard to benefit the mankind. 【Background】 Caspases(cysteine-aspartic proteases) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. The cascade reaction caused by caspases is a central part of the apoptotic process, and its activation mainly includes the mitochondria-dependent pathway and the death receptor-mediated signal transduction pathway. The downstream caspases are then activated, leading to apoptosis by cleavage of specific substrates. Inhibition of apoptotic function will lead to…
TraKine™ Pro Mitochondrion live-cell Fluorescence Probe-I just want to surpass MitoTracker®！
【REVIEW】： TraKine™ Pro Live-cell Fluorescence Tool-Perfect compatibility with long-term super-resolution fluorescence imaging TraKine™ Pro Live-cell Tubulin Staining kit-Your Best Choice for Cytoskeleton Study Living cell staining, super-resolution Lysosome tracing Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Mitochondria is crucial dynamic organelles that participate extensively in many important cellular processes. Its dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases. Capturing the dynamic process of subcellular organelles in living cells is crucial to the study of disease mechanism. The limitations…
