EliKine™ Human Leptin ELISA Kit: Precision Adipokine Quantification for Obesity and Metabolic Research
Leptin: The Satiety Hormone with Far-Reaching Metabolic Implications Leptin, the 16-kDa adipokine encoded by the ob gene, is the primary signal from adipose tissue communicating energy status to the hypothalamus. Beyond appetite regulation, leptin influences reproduction, immune function, bone metabolism, and insulin sensitivity. Leptin dysregulation is central to obesity, type 2 diabetes, and metabolic syndrome. EliKine™ Technology for Reliable Leptin Detection Abbkine's EliKine™ Human Leptin ELISA Kit (KTE6026) employs a two-site sandwich ELISA format with proprietary EliKine™ Streptavidin-HRP conjugates. The kit delivers high sensitivity and excellent specificity for Human Leptin with no significant cross-reactivity or interference from analogues. Broad Sample Compatibility Sample Type Compatibility Serum ✓ Plasma ✓ Cell Culture Supernatants ✓ Other Biological Fluids ✓ Complete Kit – Ready to Use…
EliKine™ Human IL-23 ELISA Kit: Accurate Quantification of the Th17 Polarization Master Switch
IL-23: The Cytokine Driving Autoimmunity and Chronic Inflammation Interleukin-23 (IL-23) is a heterodimeric cytokine composed of p19 and p40 subunits. It is a master regulator of Th17 cell differentiation and maintenance, driving the production of IL-17A, IL-17F, and other pro-inflammatory cytokines. IL-23 is centrally implicated in psoriasis, inflammatory bowel disease, multiple sclerosis, and rheumatoid arthritis—making it a prime therapeutic target. EliKine™ Technology for Superior Sensitivity Abbkine's EliKine™ Human IL-23 ELISA Kit (KTE6025) employs a two-site sandwich ELISA with proprietary EliKine™ Streptavidin-HRP conjugates for enhanced signal detection. The kit delivers high sensitivity and excellent specificity for Human IL-23 with no significant cross-reactivity or interference from analogues. Broad Sample Compatibility Sample Type Compatibility Serum ✓ Plasma ✓ Cell Culture Supernatants ✓ Other Biological Fluids…
EliKine™ Human IL-22 ELISA Kit: Precision Cytokine Detection for Inflammation and Barrier Immunity Research
IL-22: The Dual-Function Cytokine at the Heart of Inflammation Interleukin-22 (IL-22) is a member of the IL-10 family of cytokines, produced by NK cells and Th1-type T cells[reference:11]. It plays a dual role in inflammation—promoting tissue repair and antimicrobial defense at barrier surfaces while also driving pathological inflammation in autoimmune diseases like psoriasis and inflammatory bowel disease[reference:12]. Accurate IL-22 quantification is essential for understanding these opposing functions. EliKine™ Technology: Proprietary Streptavidin-HRP for Superior Signal Abbkine's EliKine™ Human IL-22 ELISA Kit (KTE6024) leverages proprietary EliKine™ Streptavidin-HRP conjugates for enhanced signal-to-noise ratio[reference:13]. The two-site sandwich ELISA format ensures specific capture and detection of IL-22[reference:14]. Exceptional Sensitivity: Detect Down to 8 pg/mL Parameter Specification Calibration Range 15.63 – 1000 pg/mL[reference:15] Limit of Detection 8 pg/mL[reference:16]…
Human TNFAIP8L2 ELISA Kit: Unlocking the Immune Regulation Puzzle with Precision
TNFAIP8L2: The Immune Checkpoint You Need to Measure Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2), also known as TIPE2, is a critical negative regulator of inflammation and immune homeostasis[reference:5]. It maintains T-cell receptor signaling and prevents autoimmune pathology. Dysregulated TNFAIP8L2 expression has been linked to inflammatory diseases, autoimmune disorders, and cancer immune evasion. Quantifying TNFAIP8L2 accurately in patient samples has been challenging—until now. Sandwich ELISA Format with Unmatched Specificity Abbkine's Human TNFAIP8L2 ELISA Kit (KTE60230) employs a two-site sandwich ELISA with a TNFAIP8L2-specific antibody pre-coated onto the microplate[reference:6]. After capturing the target, a biotin-conjugated detection antibody and Streptavidin-HRP generate a colorimetric signal proportional to TNFAIP8L2 concentration[reference:7]. No Cross-Reactivity, No Compromise The kit demonstrates no significant cross-reactivity or interference between Human TNFAIP8L2 and…
Human OPG ELISA Kit: Precision Quantification of the Master Osteoclast Regulator
OPG: The Bone Metabolism Biomarker You Can't Afford to Misquantify Osteoprotegerin (OPG) is a secreted cytokine that functions as a decoy receptor for RANKL, blocking osteoclast differentiation and bone resorption[reference:0]. Its dysregulation is implicated in osteoporosis, rheumatoid arthritis, cancer metastasis, and cardiovascular calcification. Accurate OPG quantification is therefore indispensable for bone biology, oncology, and metabolic research. Yet many commercial OPG ELISA kits suffer from cross-reactivity with TNFRSF11B homologs, narrow dynamic ranges, and inconsistent lot-to-lot performance. Superior Specificity: No Cross-Reactivity, No False Signals Abbkine's Human OPG ELISA Kit (KTE60224) employs a two-site sandwich ELISA format with a monoclonal antibody specific for TNFRSF11B pre-coated onto the microplate[reference:1]. The kit demonstrates no significant cross-reactivity or interference between Human TNFRSF11B and its analogues[reference:2]—a critical advantage over generic kits…
CheKine™ Micro Peroxidase Activity Assay Kit: Precise POD Detection for Oxidative Stress and Plant Physiology Research
POD: The Antioxidant Sentinel You Need to Measure Accurately Peroxidase (POD) is a cornerstone enzyme for unraveling cellular metabolism, oxidative stress responses, and enzymatic pathways across diverse biological samples[reference:139]. Accurate quantification of POD activity is essential for understanding plant stress tolerance, oxidative damage in disease models, and enzyme inhibitor screening[reference:140]. Colorimetric Detection – Simple, Sensitive, Specific KTB1150 leverages POD's ability to catalyze the oxidation of specific substrates by H₂O₂, producing colored substances with characteristic light absorption at 470 nm[reference:141][reference:142]. This mechanism ensures exceptional specificity, minimizing interference from other enzymes or matrix components[reference:143]. Microscale Format – 48 Tests Parameter Specification Tests 48T/48S[reference:144] Sample Types Plant tissues, bacteria, fungus, cells[reference:145] Readout Wavelength 470 nm[reference:146] Price $49 for 48 tests[reference:147] Unmatched Value KTB1150 offers unparalleled…
CheKine™ Micro Plant PPO Activity Assay Kit: Unlocking Plant Stress Responses and Food Quality Control
PPO: The Enzyme Behind Plant Browning, Stress Response, and Defense Polyphenol oxidase (PPO) is a core enzyme in plant physiology, mediating critical processes including wound response, pathogen resistance, and enzymatic browning[reference:124]. Accurate quantification of PPO activity is indispensable for studies in plant stress biology, post-harvest technology, and crop improvement[reference:125]. Traditional assay methods are often limited by low specificity, high sample volume requirements, and susceptibility to interference from secondary plant metabolites[reference:126]. Plant-Optimized Microscale Design The CheKine™ Micro Plant PPO Activity Assay Kit (KTB1140) addresses these limitations through a micro-scale design optimized for plant samples, leveraging a highly specific detection mechanism that minimizes cross-reactivity[reference:127]. This specificity is particularly valuable in complex plant matrices, where non-target compounds can otherwise confound results[reference:128]. Performance and Affordability…
CheKine™ Micro PFK Activity Assay Kit: Mastering the Rate-Limiting Enzyme of Glycolysis
PFK: The Tollbooth of Glycolysis – Now Measurable with Precision 6-Phosphofructokinase (PFK)—the rate-limiting enzyme of glycolysis—stands as a critical biomarker for metabolic disorders, cancer progression, and cellular stress responses[reference:107]. Its activity levels directly reflect glycolytic flux[reference:108]. Yet traditional PFK activity assays suffer from low sensitivity, interference from cellular metabolites, and cumbersome protocols[reference:109]. Abbkine's CheKine™ Micro PFK Activity Assay Kit (KTB1124) emerges as a transformative solution[reference:110]. NADH-Coupled Reaction System PFK catalyzes the phosphorylation of fructose-6-phosphate (F6P) to fructose-1,6-bisphosphate (F1,6BP) using ATP[reference:111]. KTB1124 overcomes the challenge of measuring this reaction directly by coupling it to a secondary enzymatic cascade: F1,6BP is converted to G3P, and G3P is oxidized with NAD⁺ reduced to NADH[reference:112]. Absorbance of NADH at 340 nm is proportional to PFK activity[reference:113]. Proprietary…
CheKine™ Micro PEPC Activity Assay Kit: The Gold Standard for Carbon Metabolism Research
PEPC: The Carbon-Fixing Enzyme That Demands Precision Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is a critical enzyme in carbon fixation, anaplerotic metabolism, and plant stress responses[reference:90]. Yet for decades, scientists have struggled with cumbersome PEPC assays that demand large sample volumes and deliver high background noise[reference:91]. NADH-Coupled Colorimetric Protocol The CheKine™ Micro PEPC Activity Assay Kit (KTB1122) leverages the specific catalytic conversion of PEPC, seamlessly linking the PEPC reaction to the oxidation of NADH for precise quantification by monitoring the decrease in absorbance at 340 nm[reference:92][reference:93]. Broad Sample Compatibility Sample Type Compatibility Animal Tissues ✓[reference:94] Plant Tissues ✓[reference:95] Cells (Adherent/Suspension) ✓[reference:96] Serum / Plasma ✓[reference:97] Bacteria ✓[reference:98] Competitive Advantages Feature KTB1122 Traditional Methods Sample Volume Microscale[reference:99] Large Processing Time Minutes[reference:100] Hours CO₂ Interference…
CheKine™ Micro Pyruvate Acid Assay Kit: Microscale Precision for Glycolytic Metabolite Quantification
Pyruvate: The Metabolic Hub You Can't Afford to Misquantify Pyruvate acid (PA), the end product of glycolysis and gateway to the TCA cycle, sits at the epicenter of cellular energy metabolism[reference:76]. Its concentration reflects the balance between glycolytic flux, mitochondrial respiration, and biosynthetic pathways[reference:77]. Yet as research shifts toward single-cell models, organoids, and rare clinical specimens, traditional PA assays falter—demanding excessive sample volumes and lacking sensitivity[reference:78]. DNPH-Based Colorimetric Design – Enhanced by Proprietary Stabilizer The CheKine™ Micro Pyruvate Acid (PA) Assay Kit (KTB1121) redefines PA quantification through a microscale, colorimetric design[reference:79]. PA reacts with DNPH in acidic conditions to form a yellow-orange hydrazone, measured at 450 nm[reference:80]. Microscale Engineering – 5–10 µL Sample Reactions proceed in 96-well plates with just 5–10 µL…
