Human MPP7 ELISA Kit — Product Review & Buying Guide for Epithelial Polarity and Skeletal Biology

MPP7: The 65-kDa Polarity Scaffold Hiding in Plain Sight

Membrane Palmitoylated Protein 7 (MPP7) , also known as MAGUK p55 subfamily member 7, is a ~65–80 kDa palmitoyl-modified MAGUK-family scaffold whose N-terminal PDZ domain grabs polarity determinants (LIN-7/VLP, indirectly CRB3-PALS1), whose SH3 domain mediates protein–protein wiring, and whose GUK-like domain (catalytically dead) tethers it into the DLG1 (SAP97/disc-large) complex at sites of epithelial cell–cell contact[reference:12].

When MPP7 is present and correctly localized, the epithelium knows up from down, apical from basal, sealed from leaky. When it drops or delocalizes, the cell may still express E-cadherin — but it no longer knows which direction to send a vesicle, where to build a junction, or when to stop proliferating[reference:13].

The Human MAGUK p55 subfamily member 7 (MPP7) ELISA Kit (KTE61556) from Abbkine is the reagent that finally lets you measure this polarity linchpin as a calibrated, two-site sandwich ELISA readout (ng/mL), so your EMT, kidney injury, or intestinal-barrier paper rests on a number — not a "ZO-1 looks discontinuous in the IF photo"[reference:14].

What's in the Box?

A complete, ready-to-use kit.

Advantages

1. Exceptional Specificity

The MAGUK p55 subfamily is structurally conserved — MPP1-7 share PDZ, SH3, and GUK-like domains, and generic kits targeting conserved domains light up multiple family members. The KTE61556 solves this with a pair of highly specific antibodies that recognize non-palmitoylated, unique epitopes on human MPP7 — epitopes not shared with other MAGUK p55 subfamily members or polarity-associated proteins such as DLG1. This ensures the kit exclusively detects soluble MPP7 fragments (the primary form in plasma, serum, and cell culture supernatants) and does not cross-react with membrane-bound MPP7 complexes or homologous MAGUK proteins[reference:15].

2. Low-Abundance Sensitivity

MPP7 exists primarily as a palmitoylated membrane-bound form with low-abundance soluble fragments in biological fluids. The kit's biotin-conjugated detection antibody and Streptavidin-HRP amplification system is optimized for low-abundance antigen detection — critical for MPP7 research, where soluble protein concentrations are inherently low[reference:16].

3. Validated Standard Curve

The kit features a validated standard curve with R²=0.985, ensuring accurate and reproducible quantification[reference:17].

4. Broad Dynamic Range

The kit covers a dynamic range of 0.156–10 ng/mL, spanning both low-abundance physiological levels and pathologically elevated concentrations[reference:18].

5. Multiple Sample Types

Validated for serum, plasma, cell culture supernatants, and other biological fluids — versatile enough for most experimental setups.

6. Complete Kit

All components included — no hidden costs or last-minute reagent sourcing.

Disadvantages

1. Human-Specific

This kit is specific to human MPP7 — not validated for mouse, rat, or other species.

2. Research Use Only

Not intended for human or clinical diagnosis.

3. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours.

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study epithelial cell polarity, tight junction biogenesis, or cell–cell adhesion
• Research epithelial-mesenchymal transition (EMT) and cancer metastasis
• Investigate kidney injury and intestinal barrier function
• Work on skeletal biology and bone mineral density regulation
• Need to quantify human MPP7 in serum, plasma, or cell culture supernatants
• Value specificity — no cross-reactivity with other MAGUK family members
• Want a complete, ready-to-use kit with a validated standard curve (R²=0.985)

❌ Consider alternatives if you:

• Need to detect mouse or rat MPP7 — check Abbkine's other offerings
• Are working with clinical diagnostic samples
• Require a faster assay (<3 hours)

Quick Specs Summary

📌 Reference Link: Human MAGUK p55 subfamily member 7 (MPP7) ELISA Kit — KTE61556