Bcl-2 Monoclonal Antibody (ABM0010): Precision Detection for Apoptosis Research
The Bcl-2 Homolog Problem: Why Cross-Reactivity Derails Apoptosis Studies
Apoptosis research hinges on dissecting the Bcl-2 family's delicate balance—where overexpression drives cancer cell survival and underexpression triggers neurodegeneration. Yet this field is plagued by a paradox: while Bcl-2 is one of the most studied apoptosis regulators, many labs struggle with antibodies that fail to distinguish Bcl-2 from its homologs (Bcl-xL, Mcl-1) or provide inconsistent results across assays[reference:23].
Epitope Precision: The BH4 Domain Advantage
Abbkine's Bcl-2 Monoclonal Antibody (ABM0010) redefines this landscape. Unlike polyclonals that risk cross-reactivity, ABM0010—a mouse-derived IgG1κ—targets a unique linear epitope in the BH4 domain of human Bcl-2 (residues 20–40), a region absent in other Bcl-2 family members[reference:24]. This strategic epitope selection ensures >99% specificity for Bcl-2[reference:25].
Validated Performance Across Multiple Assays
| Application | Recommended Dilution | Performance |
|---|---|---|
| Western Blot | 1:1000–1:2000 | Clean band at ~26 kDa |
| Immunofluorescence | 1:50–1:200 | Precise subcellular localization |
| IHC-P | 1:200 | Reliable tissue staining |
| Flow Cytometry | Validated | Accurate quantification |
| Proximity Ligation Assay (PLA) | Validated | Protein interaction studies |
Cited in Peer-Reviewed Research
ABM0010 has been cited in multiple publications, including British Journal of Nutrition, where it was used to investigate the mechanistic role of Chrysin in protecting against cardiac hypertrophy and fibrosis in rats[reference:26].
Head-to-Head: Bcl-2 Antibody Comparison
| Feature | ABM0010 | Generic Competitors |
|---|---|---|
| Clonality | Monoclonal (IgG1κ) | Polyclonal (variable) |
| Epitope | BH4 domain (aa 20–40) | Often conserved regions |
| Bcl-xL Cross-reactivity | None observed | Often present |
| Mcl-1 Cross-reactivity | None observed | Often present |
| Applications | WB, IF, IHC-P, Flow, PLA | Variable |
Product Specifications
| Parameter | ABM0010 |
|---|---|
| Target | Bcl-2 |
| Clone | Monoclonal (Mouse IgG1κ) |
| Immunogen | Synthetic peptide from Bcl-2 BH4 domain (aa 20–40) |
| Applications | WB, IF, IHC-P, Flow Cytometry, PLA |
| Molecular Weight | ~26 kDa |
| Reactivity | Human |
The Bottom Line
For apoptosis researchers demanding unambiguous Bcl-2 detection without confounding signals from homologs, ABM0010 delivers the epitope precision, validation rigor, and multi-assay versatility that apoptosis research demands.
📎 Reference: Bcl-2 Monoclonal Antibody (ABM0010)
