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Caspase(1,2,3,4,6,8,9) Assay Kit- Simple and convenient, to boost your apoptosis study

【Tips】If you are studying whether a certain drug can trigger apoptosis which relys on the caspase related pathway, and want to detect the activity of caspase, this article is suitable for you! This article is especially fit for researchers who are working hard to benefit the mankind.

【Background】

Caspases(cysteine-aspartic proteases) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation. The cascade reaction caused by caspases is a central part of the apoptotic process, and its activation mainly includes the mitochondria-dependent pathway and the death receptor-mediated signal transduction pathway. The downstream caspases are then activated, leading to apoptosis by cleavage of specific substrates. Inhibition of apoptotic function will lead to tumorigenesis and abnormal immune function. However, hyperfunction of apoptotic function is closely related to the pathogenesis of tissue damage and organ failure.

【Caspase Structure】

【Assay Principle】

Abbkine Caspase Assay Kits are based on different caspases can catalyze specific substrates to generate pNA(p-nitroaniline), resulting in the release of p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.

Product name Cat#
Caspase-1 Assay Kit (Colorimetric) KTA3020
Caspase-2 Assay Kit (Colorimetric) KTA3021
Caspase-3 Assay Kit (Colorimetric) KTA3022
Caspase-4 Assay Kit (Colorimetric) KTA3023
Caspase-6 Assay Kit (Colorimetric) KTA3024
Caspase-8 Assay Kit (Colorimetric) KTA3025
Caspase-9 Assay Kit (Colorimetric) KTA3026

【Assay Procedure】

  1. Add samples, background control and standard
  2. Add Reaction Buffer and substrate, incubate
  3. Read samples at 405 nm

Simple three steps, a big step in scientific research!

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.