|Product name||Caspase-4 Assay Kit (Colorimetric)|
|Applications notes||Caspase 4 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-LEVD-pNA (acetyl-Leu-Glu-Val-Asp p-nitroanilide) by caspase 4, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.|
|Kit components||• Cell Lysis Buffer
• Reaction Buffer (2×)
• Ac-LEVD-pNA (4mM)
• DTT (100×)
|Features & Benefits||• Simple and optimized procedure.
• Fast and convenient.
• Detect early/middle stages of apoptosis.
|Storage instructions||Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-4 (also known as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. The active caspase-4 is a heterotetramer consisting of two large and two small subunits.|
Fig. Typical data of pNA standard curve using Abbkine Caspase-4 Assay Kit (Colorimetric).
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