Live-cell imaging technique allows real-time examination of almost every aspect of cellular function under normal and experimental conditions, so it is especially popular with cytology researchers. Abbkine TraKine™ Pro is a series of cell-permeable organic fluorescent probes which can selectively recognize and label organelles. Currently, we launched the staining kits involve tubulin, lysosome, mitochondrion and nuclei. This paper introduces the TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution).
Function of nucleus
The nucleus is the largest and most important cell structure in eukaryotic cells, mainly composed of nuclear envelope, chromatin, nuclear skeleton, nucleolus and nucleosome. Nuclear membrane is a two-layer membrane that completely encloses the nucleus and separates the material within the membrane from the cytoplasm and lamina.
The nucleus contains most of the cell’s genome, organized as multiple long linear DNA molecules in a complex with a large variety of proteins, such as histones, to form chromosomes. It is the regulatory center of cell heredity and metabolism, and plays an important role in cell metabolism, growth and differentiation.
Commercially available nucleus fluorescent probes
The nucleus is the place where the genetic material is stored and reproduced, and it is also the control center of cellular heredity and cell metabolism. Studying its function, mechanism, dynamics and interaction is important direction for cytology researchers.
At present, the existing nuclear dyes (such as PI, DAPI, ethd-1, etc.) are mostly used for dying cell staining, apoptosis research, nuclear counterstaining, and so on. Hoechst dye is a compound synthesized by Hoechst AG originally, which is soluble in water and organic solvents. It can cross cell membranes and bind to living or fixed cells, the fluorescence intensity enhanced along with the increase of the pH value of the solution.
|DAPI||BMD00063||358/461 (with DNA)||Dead cell staining; counterstaining; Apoptosis study|
|Hoechst 33258||BMD00061||352/461 (with DNA)||Live or fixed cells; Apoptosis study|
|Hoechst 33342||BMD00062||350/461 (with DNA)||Live cells or tissues; fixed cells or tissue; Apoptosis study|
|Ethidium Homodimer-1 (EthD-1)||BMD00060||528/617 (with DNA)||Dead cell staining; High affinity fluorescent nucleic acid dyes, binding DNA or RNA|
However, there is no dye that can be used to observe the dynamics of living cell nucleus very well currently. Abbkine researchers developed a unique TraKine™ Pro fluorescent probe that can track the 3d dynamics of living cell nucleus for a long time with ultra-high resolution.
The TraKine™ Pro nuclear fluorescence probe has been specially optimized, which contains a fluorescent dye and a small molecular group that specifically identifies the nucleus, to locate the nucleus and track its dynamics and changes. It is the only highly specific live cell nuclear staining product on the market.
|Product name||Cat#||Ex/Em（nm）||Live or fixed cells||Microscope compatibility|
|TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution)||KTC4510||650/665||Live& fixed cells||Fluorescence microscope，Confocal, SIM, PALM, STORM, TIRF|
【Features & Benefits】
1 Optimized staining protocol for labeling subcellular structures in mammalian living and fixed cells.
2 Super resolution, especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
3 Fluorescence can last for several hours in cells, stable and persistent, especially ideal for monitoring dynamics
4 High specificity, low background, excellent photostability and good cell permeability.
5 Safe, low levels of cytotoxicity to cells
【Perfect results are the best illustration】
|KTC4510 SIM imaging: U2OS cells||KTC4510 Confocal imaging: U2OS cells|
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.