|Product name||TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution)|
|Applications notes||TraKine™ Pro is series of long-term super-resolution cell staining imaging portfolio for labeling subcellular structures of live and fixed cells. TraKine™ Pro proprietary excellent ﬂuorescent dyes span the full UV-visible and near IR spectrum. Customized products based on TraKine™ Pro technology are also available. TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Nuclei in mammalian living cells and fixed cells with high specificity and low background. The proprietary probe in the kit consists of a Nucleus recognition unit and a deep red fluorescent dye (Ex/Em=650/665 nm), the recognition unit can selectively recognize Nuclei and binding with it. It is especially suitable for long-term super-resolution imaging.|
|Kit components||• NucleiRed™ (200 uM)
• Buffer N
|Features & Benefits||• Optimized staining protocol for labeling Nuclei in mammalian living and fixed cells. The product has been tested in U2OS, Hela, COS-7 and ARPE cell lines. If the sample type is not included in the above cell lines, we can provide experimental services for specific cell lines.
• Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM).
• Proprietary NucleiRed™ (Ex/Em = 650/665nm)-high specificity, low background and excellent photostability.
• Low levels of cytotoxicity.
|Usage notes||Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.|
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Nucleus is a membrane bound structure that contains the cell’s hereditary information and controls the cell’s growth and reproduction. Nucleus is present in all eukaryotic cells, they may be absent in few cells like the mammalian RBCs. Nucleus controls the heredity characteristics of an organism. It main cellular metabolism through controlling synthesis of particular enzymes. It is responsible for protein synthesis, cell division, growth and differentiation. It also regulates the integrity of genes and gene expression.|
Fig.1. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.
Fig.2. SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution). It can be seen that the kit can specifically label Nuclei structures in living cells and perform ultra-high resolution imaging.