By in Product release

Want High accuracy and specificity? Yes, it’s Abbkine CheKine™ Catalase (CAT) Activity Assay Kit with great value

【Background】

Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four iron-containing heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum pH for human catalase is approximately and has a fairly broad maximum, the rate of reaction does not change appreciably between pH 6.8 and 7.5. The pH optimum for other catalases varies between 4 and 11 depending on the species. The optimum temperature also varies by species.

【Abbkine Catalase Activity Assay Kit

Abbkine Catalase Activity Assay Kit utilizes the peroxidatic function of catalase for measuring


catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.

Most commercial Catalase Activity Assay Kits apply this principle that residual hydrogen peroxide is oxidized by peroxidase to form the colored substrate, and the catalase activity was detected indirectly. However, other peroxidases in the sample may interfere with the experimental results and this method may cause standard curve errors due to unstable hydrogen peroxide.

Abbkine kit has unique advantages, which can detect the enzyme activity directly, with better more veracity and higher specificity. Since methanol is a unique substrate of catalase, other peroxidases cannot use methanol as a substrate, so interference signals are avoided.

Product name Cat# Detection Range
CheKine™ Catalase (CAT) Activity Assay Kit KTB1040 2-75 µM

【Kit components

• Assay Buffer (10x)

• Sample Diluent (10x)

• Formaldehyde standard (4.25 M)

• Catalase (positive control)

• Potassium Hydroxide

• Hydrogen Peroxide

• Chromogen

• Potassium Periodate

【Features & Benefits

  • Determination of catalase activity in serum, plasma, tissue/cell lysates and other biological fluids.
  • A broad range linearity of 2-75 µM, measure catalase activity down to 2 U/ml
  • Determining catalase activity directly by utilizing the peroxidase function of catalase, which cannot be interfered by other peroxidases. 
  • Simple operation, only four steps to complete the measurement

【Results Analysis

Formaldehyde (µM)
  1. Calculate the formaldehyde concentration of the samples using the equation obtained from standard

2)Calculate the CAT activity of the sample using the following equation. One unit is defined as the amount of enzyme that will cause the formation of 1.0 nmol of formaldehyde per minute at 25°C

Note: µM means nmol/ml

Please refer to Product datasheet: https://www.abbkine.com/file/booklet/KTB1040-B.pdf

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.