- Product name
Caspase-6 Assay Kit (Colorimetric)
- Application notes
Caspase 6 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-VEID-pNA (acetyl-Val-Glu-Ilel-Asp p-nitroanilide) by caspase 6, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.
- Kit components
• Cell Lysis Buffer
• Reaction Buffer (2×)
• Ac-VEID-pNA (4mM)
• DTT (100×)
- Features & Benefits
• Simple and optimized procedure.
• Fast and convenient.
• Detect early/middle stages of apoptosis.
- Storage instructions
Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-6 (also known as Mch2) is a member of the caspase-family of cysteine proteases. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and genomic maintenance.
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Caspase-6 Assay Kit (Colorimetric)
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Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact firstname.lastname@example.org for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.