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Caspase-9 Assay Kit (Colorimetric)

Caspase-9 Assay Kit (Colorimetric)

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Product name Caspase-9 Assay Kit (Colorimetric)
Applications notes Caspase 9 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-LEHD-pNA (acetyl-Leu-Glu-His-Asp p-nitroanilide) by caspase 9, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.

Product Properties

Kit components • Cell Lysis Buffer
• Reaction Buffer (2×)
• Ac-LEHD-pNA (4mM)
• pNA(10mM)
• DTT (100×)
Features & Benefits • Simple and optimized procedure.
• Fast and convenient.
• Detect early/middle stages of apoptosis.
Storage instructions Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-9 (also known as ICE-LAP6 and Mch6) is a member of the caspase-family of cysteine proteases. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis.

Image & description

Fig. Typical data of pNA standard curve using Abbkine Caspase-9 Assay Kit (Colorimetric).

Fig. Typical data of pNA standard curve using Abbkine Caspase-9 Assay Kit (Colorimetric).

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