Abbkine Human MAGUK p55 Subfamily Member 7 (MPP7) ELISA Kit (KTE61556): A Precision Practical Guide for Quantitative MPP7 Detection in Epithelial and Skeletal Biology Research
Membrane palmitoylated protein 7 (MPP7), a key member of the MAGUK p55 Stardust family, is a non-negotiable molecular player in orchestrating epithelial cell polarity, tight junction biogenesis and site-specific bone mineral density regulation—making its accurate quantitative detection a foundational step for research spanning cell biology, skeletal physiology and tissue repair. As a membrane-associated guanylate kinase (MAGUK) protein, MPP7 exists primarily as a palmitoylated membrane-bound form with low-abundance soluble fragments in biological fluids and cell culture supernatants; compounding this challenge, the high structural homology among MAGUK family members creates a risk of cross-reactivity in non-optimized detection assays. For researchers seeking reliable, specific quantification of human MPP7, the Abbkine Human MAGUK p55 subfamily member 7 (MPP7) ELISA Kit (Cat. No. KTE61556) (product link: https://www.abbkine.com/product/human-maguk-p55-subfamily-member-7-mpp7-elisa-kit-kte61556/) emerges…
Abbkine Collagen I Mouse Monoclonal Antibody (4H10) (ABM40379): A Gold-Standard Tool for Type I Collagen Research Across Biological Disciplines
Type I Collagen, encoded by the COL1A1 gene, is the most abundant fibril-forming collagen in the human body and the structural backbone of nearly all connective tissues, including bone, dermis, tendon, and cornea. Its biological significance extends far beyond structural support: mutations in COL1A1 are linked to a spectrum of heritable connective tissue disorders such as osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis, while its dysregulated deposition and remodeling drive pathological processes in cancer stroma formation, tissue fibrosis, and chronic wound healing. For researchers in skeletal biology, tumor microenvironment (TME) research, regenerative medicine, and clinical pathology, the specific and sensitive detection of endogenous Type I Collagen is an indispensable experimental step—yet the field has long struggled with key technical challenges,…
Abbkine PDGFRα Mouse Monoclonal Antibody (7A3) (ABM40334): A Versatile Tool for PDGFRα Research Across Translational and Clinical Science
Platelet-derived growth factor receptor alpha (PDGFRα) is a cell surface tyrosine kinase receptor that’s a linchpin for mesenchymal cell proliferation, organ development, and wound healing—yet its dysregulation and mutation are also driving forces behind a host of human cancers, including gastrointestinal stromal tumors (GIST), non-small cell lung cancer, and gliomas, as well as rare disorders like idiopathic hypereosinophilic syndrome. For researchers spanning cancer biology, developmental biology, and translational medicine, reliable detection of endogenous PDGFRα is non-negotiable—but the field has long grappled with common frustrations: antibodies that cross-react with PDGFRβ, weak signal in formalin-fixed paraffin-embedded (FFPE) tissue, poor cross-species performance, and high non-specific staining in immunofluorescence (IF). The Abbkine PDGFRα Mouse Monoclonal Antibody (7A3) (Cat. No. ABM40334) (product link: https://www.abbkine.com/product/pdgfr%ce%b1-mouse-monoclonal-antibody-7a3-abm40334/) cuts through these pain…
Abbkine GFAP Monoclonal Antibody (ABM0021): Practical Guide for Astrocyte Marker Detection
Glia Fibrillary Acidic Protein (GFAP) is the gold-standard marker for mature astrocytes, central to CNS homeostasis, neuroinflammation, and neurodegenerative disease pathogenesis. Reliable endogenous GFAP detection is critical for neurobiology and neuropathology research, yet the field faces pitfalls like poor cross-species reactivity, inconsistent application performance, and vague optimization guidelines. The Abbkine GFAP Monoclonal Antibody (Cat. No. ABM0021) (product link: https://www.abbkine.com/product/gfap-monoclonal-antibody-abm0021/) solves these issues: a mouse-derived, affinity-purified antibody validated for human, rat, and mouse GFAP across Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF). This condensed practical guide focuses on application-specific optimization, antibody handling, and troubleshooting to help researchers generate reproducible, publication-quality results. WB Optimization for Abbkine GFAP Monoclonal Antibody ABM0021 The Abbkine GFAP Monoclonal Antibody ABM0021 is validated for WB with a…
Abbkine PINK1 Polyclonal Antibody (ABP59917): A Precision Tool for Mitochondrial Biology and Parkinson’s Disease Research
PTEN-induced putative kinase protein 1 (PINK1), a mitochondrial-localized serine/threonine kinase, stands as a central player in cellular homeostasis—governing mitochondrial quality control, mitigating stress-induced mitochondrial dysfunction, and acting as a critical genetic determinant of autosomal recessive early-onset Parkinson’s disease (PD). Mutations in the PINK1 gene disrupt mitochondrial repair and clearance pathways, driving neuronal loss in the substantia nigra and contributing to the pathogenesis of PD and other neurodegenerative disorders linked to mitochondrial dysfunction. For researchers across neurobiology, mitochondrial biology, and translational neurodegenerative disease research, the specific and sensitive detection of endogenous PINK1 protein is a prerequisite for unraveling its mechanistic roles and validating its potential as a therapeutic target. The Abbkine PINK1 Polyclonal Antibody (Cat. No. ABP59917) (product link: https://www.abbkine.com/product/pink1-polyclonal-antibody-abp59917/) is engineered to address the core…
Abbkine MARCO Polyclonal Antibody (ABP59218): A Definitive Tool for Innate Immunity and Macrophage Biology Research
Macrophage Receptor With Collagenous Structure (MARCO), a key member of the class A scavenger receptor family, stands as a cornerstone of the mammalian innate antimicrobial immune system and a critical marker of functional macrophage biology. As a cell surface receptor predominantly expressed on macrophages, MARCO mediates the recognition and binding of both Gram-negative and Gram-positive bacteria via its extracellular scavenger receptor cysteine-rich (SRCR) domain, orchestrating early immune responses to microbial infection, regulating inflammatory signaling, and maintaining tissue immune homeostasis. Beyond antimicrobial immunity, MARCO is increasingly implicated in disease pathogenesis—from infectious disorders and chronic inflammation to tumor microenvironment remodeling and autoimmune diseases—making its precise detection and quantification indispensable for cutting-edge research across immunology, microbiology, and oncology. The Abbkine MARCO Polyclonal Antibody (Cat.…
Abbkine Human Double-Strand Break Repair Protein MRE11A (MRE11A) ELISA Kit (KTE61540): A Pivotal Tool for DNA Damage Repair and Genomic Stability Research
MRE11A stands as a core nuclear protein at the heart of eukaryotic genomic maintenance, orchestrating DNA double-strand break (DSB) repair, homologous recombination, telomere length homeostasis, and DNA damage signaling—with its dysfunction linked to Ataxia-Telangiectasia-like disorder (ATLD), increased cancer susceptibility, and accelerated cellular senescence. As research into DNA damage repair (DDR) pathways expands into oncology, genetic disease, and aging research, the accurate and specific quantification of human MRE11A has become a non-negotiable prerequisite for unraveling mechanistic insights and translating discoveries into clinical applications. The Abbkine Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit (Cat. No. KTE61540) (product link: https://www.abbkine.com/product/human-double-strand-break-repair-protein-mre11a-mre11a-elisa-kit-kte61540/) is engineered as a human-specific, quantitative solution to the unique technical challenges of MRE11A detection, addressing longstanding industry pain points and emerging as a…
Abbkine Human Melatonin (MT) ELISA Kit (KTE61518): Redefining Ultra-Sensitive Detection for the Multifaceted "Sleep Hormone"
Melatonin (MT) isn’t just the go-to molecule for sleep research—this small indoleamine is a biological workhorse, governing circadian rhythm homeostasis, modulating oxidative stress, boosting immune function, and even playing emerging roles in oncology, neurology, and metabolic health. But here’s the catch for every researcher studying human MT: this hormone circulates in bodily fluids at ultra-low physiological concentrations, degrades in minutes at room temperature, and breaks down when exposed to light. For years, the field has been stuck with either overly complex, high-cost detection methods (like LC-MS/MS) that require specialized gear, or generic ELISA kits with poor sensitivity that can’t capture real physiological MT levels. That’s why the Abbkine Human Melatonin (MT) ELISA Kit (Cat. No. KTE61518) (product link: https://www.abbkine.com/product/human-melatonin-mt-elisa-kit-kte61518/) has become a game-changer for…
Abbkine Human Myosin Regulatory Light Chain 12B (MYL12B) ELISA Kit (KTE61444): A Comprehensive Practical Guide for Quantitative MYL12B Detection
Myosin regulatory light chain 12B (MYL12B) is a critical calcium-binding protein encoded by the MYL12B gene, playing pivotal roles in semaphorin signaling pathways, tight junction formation, and cytoskeletal dynamics—core cellular processes underlying cell migration, adhesion, and tissue homeostasis. As a paralog of MYL12A, MYL12B shares structural homology with its isoform, creating inherent challenges for specific detection; meanwhile, its calcium-binding property renders the protein structurally labile, complicating accurate quantification in biological samples. For researchers in cell biology, developmental biology, and translational biomedical research, the Abbkine Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit (Cat. No. KTE61444) (product link: https://www.abbkine.com/product/human-myosin-regulatory-light-chain-12b-myl12b-elisa-kit-kte61444/) is a purpose-engineered quantitative sandwich ELISA that addresses these unique technical hurdles. This practical guide and methodology framework is tailored exclusively to this kit,…
Abbkine Human NEDD4-Binding Protein 1 (N4BP1) ELISA Kit (KTE61399): A Precision Tool for Ubiquitin Signaling and E3 Ligase Regulation Research
Ubiquitin-mediated post-translational modification stands as one of the most critical regulatory pathways in cellular biology, governing processes from protein degradation and cell apoptosis to immune response modulation—and NEDD4-binding protein 1 (N4BP1) emerges as a key modulator of this cascade, functioning as a selective inhibitor of the ITCH E3 ubiquitin-protein ligase and a conserved RNA-binding protein with deep ties to cellular signal homeostasis. For researchers investigating ubiquitin signaling, E3 ligase function, and disease-associated dysregulation of these pathways, accurate and specific quantification of human N4BP1 is not just an experimental step, but a prerequisite for generating definitive, interpretable data. Yet the field has long struggled with generic detection tools that suffer from cross-reactivity with N4BP1 paralogs (e.g., KHNYN) or NEDD4 family proteins,…
