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Easy to deal with Immunoprecipitation, "IP new weapon" is coming

Date:2022-07-28 Views:321

1.Background principle:

Immunoprecipitation refers to a method of preliminarily separating the target protein by precipitating the antigen (usually the target protein) from the mixed system by using the characteristic that the antibody can specifically bind to the antigen. Immunoprecipitation is developed based on the traditional affinity purification method.

 

2.Questions:

Although the principle is not difficult, the result is not easy. 1. How many researchers are struggling in the sea of searching for the target strips, 2. How many people are foolishly unable to distinguish the strips under the interference of light and heavy chains; 3. How many people are struggling "Lost yourself" in complex manipulation experiments.

Solution:

Abbkine has launched a series of IP toolboxes to directly address the experimental pain points encountered in IP (Co-IP), help your IP experiment process and optimize your experimental operation process.

Universal IP/Co-IP Toolkit (Magnetic Beads)-KTD104-EN

Universal IP/Co-IP Toolkit (Agarose)-KTD105-EN

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)-KTI1010-EN

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)-KTI1020-EN

 

3. Product advantages and characteristics:

Part A:

Universal IP/Co-IP Toolkit (Magnetic Beads)-KTD104-EN

Universal IP/Co-IP Toolkit (Agarose)-KTD105-EN

① Unique IPKine™ secondary antibodies to perfectly eliminate heavy chain interference.

② Efficient antibody binding capacity and low protein non-specific adsorption rate.

③ Non-Denaturing Lysis Buffer and Denaturing Lysis Buffer, meet IP/Co-IP/WB requirements.

④ IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules.

⑤ Also available in 2 different product formats (magnetic beads/agarose).

 

Part B:

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)-KTI1010-EN

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)-KTI1020-EN

① Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption.

② Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time.

③ Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference.

 

Part C:Kit Components

Components KTD104-EN KTI1010-EN KTI1020-EN
Universal IP/Co-IP Toolkit (Magnetic Beads) Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)
• Non-Denaturing Lysis Buffer 25 mL 25 mL 25 mL
• Denaturing Lysis Buffer 25 mL × ×
• 20×Wash Buffer 20 mL 20 mL 20 mL
• Protein A/G Magnetic Beads 0.45 mL 0.5 mL 0.5 mL
• Elution Buffer 2 mL 2 mL 2 mL
• Neutralization Buffer 0.2 mL 0.2 mL 0.2 mL
• 100×Proteinase Inhibitor Cocktail 0.2 mL 0.2 mL 0.2 mL
• Mouse IgG (1 mg/mL) 30 μL 30 μL ×
• Rabbit IgG (1 mg/mL) 30 μL × 30 μL
• IPKine™ HRP, Goat Anti-Mouse IgG LCS 30 μL 30 μL ×
• IPKine™ HRP, Mouse Anti-Rabbit IgG LCS 30 μL × 30 μL

 

4.Advantageous price

Product Name Catalog No Size Price/$
Universal IP/Co-IP Toolkit (Magnetic Beads) KTD104-EN 20T 169
Universal IP/Co-IP Toolkit (Agarose) KTD105-EN 20T 169
Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) KTI1010-EN 20T 139
Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit) KTI1020-EN 20T 139