Human PRKAR1B ELISA Kit — Application Scenarios for Neuroscience, Neuromuscular Disease, and Neuronal Tumor Research

Article Type: Application Scenarios — showcasing the kit's value across different research fields investigating the brain-enriched PKA regulatory subunit

PRKAR1B: The Brain's Specialized cAMP Gatekeeper

PRKAR1B (cAMP-dependent protein kinase type I-beta regulatory subunit, RIβ) is the brain-enriched, neuron-specific regulatory subunit of PKA that holds the catalytic subunits in check at postsynaptic densities, dendritic spines, and neuromuscular junctions[reference:0]. Unlike the ubiquitously expressed PRKAR1A (RIα), PRKAR1B is not a redundant backup — it is the isoform that anchors PKA at neuronal structures via interactions with AKAPs (A-kinase anchoring proteins), creating local cAMP microdomains where the kinase phosphorylates substrates that diffusion-based signaling can't reach[reference:1].

When PRKAR1B drops, relocalizes, or gets co-opted by RNA toxicity, the consequences show up as cognitive deficits, synaptic plasticity collapse, and neuromuscular degeneration[reference:2]. The Human PRKAR1B ELISA Kit (KTE61151) from Abbkine is built to give you a calibrated, two-site sandwich ELISA readout of this neuron-specific scaffold — so you're measuring it like a quantitative variable, not a "brown band on a blot that sort of looks fainter"[reference:3].

Neuroscience: Synaptic Plasticity, Learning, and Memory

PRKAR1B knockout mice show spatial learning deficits, reduced long-term potentiation (LTP) , and altered striatal dopamine signaling[reference:4]. Neuroscientists use this kit to:

• Quantify PRKAR1B in hippocampal and cortical tissue lysates
• Monitor PRKAR1B expression in synaptosomal preparations and postsynaptic density (PSD) fractions
• Study the role of PRKAR1B in cAMP/PKA signaling at synapses
• Investigate PRKAR1B dynamics in learning and memory paradigms

The kit delivers quantitative data from serum, plasma, tissue lysates, and cell culture supernatants, with a dynamic range of 0.156–10 ng/mL and sensitivity of ~0.05–0.09 ng/mL[reference:5].

Neuromuscular Disease Research: From Synapse to Muscle

PRKAR1B is expressed not only in the brain but also in skeletal muscle[reference:6]. Neuromuscular researchers use this kit to:

• Quantify PRKAR1B in neuromuscular junction preparations
• Study PRKAR1B's role in motor neuron disease and neuromuscular degeneration
• Investigate the effects of RNA toxicity on PRKAR1B expression and localization
• Monitor PRKAR1B as a biomarker in neuromuscular disease models

Neuronal Tumor Research: PKA Signaling in Brain Tumors

cAMP/PKA signaling is dysregulated in many neuronal tumors. Neuro-oncology researchers use this kit to:

• Quantify PRKAR1B in glioma and neuroblastoma tissue lysates
• Study the role of PRKAR1B in tumor cell proliferation and differentiation
• Investigate PRKAR1B as a prognostic biomarker in brain tumors
• Monitor PRKAR1B levels in patient plasma for translational studies

Why Choose This Kit for Your PRKAR1B Research?

Kit Components at a Glance

• Human PRKAR1B microplate (pre-coated with capture antibody)
• Human PRKAR1B standard
• Human PRKAR1B detection antibody (biotin-conjugated)
• Streptavidin-HRP
• Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate covers

🔬 Which research field does your work belong to?
Neuroscience | Neuromuscular Disease | Neuro-Oncology | Cell Signaling | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: Human CAMP-dependent protein kinase type I-beta