Your Best Choice: SuperKine™ West Pico PLUS Chemiluminescent Substrate

Background

Western Blot HR substrate series products are chemiluminescent substances used to detect HRP-labeled antibodies during Western blotting. All such substrates consist of two parts, peroxide reagent and luminol/enhancer. Mix equal volumes of the two solutions, incubate with the blotted membrane, and then use the film or imaging system to detect the results.

Chemiluminescence measurement depends on the emission of light as a product of a chemical reaction, and is usually used for protein detection in Western blotting. The secondary antibody used in the Western blot is conjugated with horseradish peroxidase (HRP) enzyme, which reacts with the HRP substrate luminol. The reaction emits light at 428 nm, and the light signal can be captured on X-ray film or a digital imager.

However, luminol emits only weakly, so an enhancer is added to the reaction to increase the signal. This enhancement of the luminol-based signal is commonly referred to as enhanced chemiluminescence (ECL).

Products Introduction

Abbkine substrate kits are value-priced,classical peroxidase substrates for enhanced chemiluminescence(ECL) that directly replaces costlier products without the need to re-optimize conditions. They are innovative and optimized based on the classic peroxidase substrate system.It is the perfect combination of excellent quality and friendly price.

1.SuperKine™ West Pico PLUS Chemiluminescent Substrate

SuperKine™ West Pico PLUS Chemiluminescent Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.

2.SuperKine™ West Femto Maximum Sensitivity Substrate

SuperKine™ West Femto Maximum Sensitivity Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.

Product Properties

1.Kit components

• ECL HRP Substrate Reagent A
• ECL HRP Substrate Reagent B.

2.Features & Benefits

Abbkine SuperLumia ECL Plus HRP Substrate Kit is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

3.Usage notes

• Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.
• Suggest 0.1-0.2ml of SuperLumia ECL Plus working solution for 1 cm2 size of blot membrane.
• Incubate blot 1-5 minutes in SuperLumia ECL Plus Substrate working solution.
• Drain excess reagent. Cover blot with clear plastic wrap.
• Expose blot to X-ray film.

4.Storage instructions

Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.

5.Shipping

Gel pack with blue ice.

6.Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific’s position: Serve the worldwide customers who do cytology and proteomics research, providing both economical and technical product solution in the manner of application process and product combination.