Protein Quantification- Reliable and Stable Choice of Excellence
Accurate protein quantification is necessary for protein-related experiments that involve research in molecular biology, cell biology, biochemistry, developmental biology, and neuroscience. Abbkine protein quantification products include Protein Quantification Kits (BCA Assay and Bradford Assay), SuperLumia ECL HRP Substrate Kit, Protein Gel Flash Staining Kit, Colorcode Prestained Protein Marker and so on. Protein Quantification Kits Featured products: 1. Protein Quantification Kits (BCA Assay) • Save time—Easier and 4x faster than traditional Lowry methods. • High sensitivity—The detection line is as low as 25ug/ml, and the minimum detection protein is 0.5ug. • Good compatibility—unaffected by most ionic and non-ionic detergents. Compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80 in the sample. • Excellent linearity-linear standard curve range:…
The third generation of fluorescent dye AbFluor™/highly water-soluble fluorescent dye
AbFluor™ dyes are a series of highly water-soluble fluorescent dyes introduced by Abbkine after many years of research, covering the ultraviolet, visible and near-infrared spectrum, suitable for labeling biological macromolecules, especially proteins and nucleic acids. Their excellent hydrophilicity enables protein-conjugated labeling to be performed easily in aqueous media, while minimizing the need for organic solvents. AbFluor™ dyes not only have better fluorescence performance than other fluorescent dyes (such as FITC, TRITC, Alexa Fluor and Dylight dyes), but also the coupling products are more stable. Why recommend AbFluor™ fluorescent dyes? With innovative modifications to the core structure of the dye, AbFluor™ dyes have more brilliant properties than other commercial dyes, including stronger fluorescent brightness, better light stability, wider pH tolerance range,…
Western Blot Product Portfolio- Sample Preparation
Overview of western blot (WB): Western Blot is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to color the cell or biological tissue samples treated by gel electrophoresis through specific antibodies. Information on the expression of specific proteins in the analyzed cells or tissues is obtained by analyzing the staining position and staining depth. Sample preparation: Good protein is half the success of WB. We need to lyse cells and tissues to extract the target protein. Once cracking occurs, hydrolysis, dephosphorylation and denaturation begin. If appropriate protease or phosphatase inhibitor is added to the cracking solution, these reactions will be greatly slowed down and the chance of extracting good protein will be increased.…
Selected primary antibody| GSK3β Mouse Monoclonal antibody recommendation
Review of Related Articles: Research Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody Abbkine P53 antibody helps basic cancer research! Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody Selected primary antibody|TNF-α antibody recommendation Glycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that has been evolutionarily very conservative.The protein encoded by GSK3β (glycogen synthase kinase 3 beta) is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. GSK-3β can also act on many signaling protein structural proteins and transcription factors, and regulate cell differentiation, proliferation, survival and apoptosis. GSK-3β is a key enzyme involved in liver glucose metabolism. It inhibits its activity by phosphorylating GS, reduces liver glucose synthesis, increases…
Selected primary antibody| Cleaved Caspase-3 antibody recommendation
Review of Related Articles: Research Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody Abbkine P53 antibody helps basic cancer research! Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody Selected primary antibody|TNF-α antibody recommendation Cleaved Caspase-3 is an Activated Form of Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene…
Selected primary antibody|TNF-α antibody recommendation
Review of Related Articles: Research Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody Abbkine P53 antibody helps basic cancer research! Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody Tumor necrosis factor (TNF, cachexin, or cachectin; once named as tumor necrosis factor alpha or TNFα) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNF is a member of the TNF superfamily, consisting of various transmembrane proteins with a homologous TNF domain. The primary role of TNF is in the regulation…
Marker of myofibroblast antibody-α-SMA/ACTA2 Monoclonal Antibody
ACTA2 (actin alpha 2) is an actin protein with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actins are a family of globular multi-functional proteins that form microfilaments. ACTA2 is one of 6 different actin isoforms and is involved in the contractile apparatus of smooth muscle. ACTA2 (as with all the actins) is extremely highly conserved and found in nearly all mammals. In humans, ACTA2 is encoded by the ACTA2 gene located on 10q22-q24. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, Moyamoya disease, and multisystemic smooth muscle dysfunction syndrome. ACTA2 (commonly referred to as alpha-smooth muscle actin or α-SMA)…
Abbkine P53 antibody helps basic cancer research!
P53 gene, human tumor suppressor gene. The gene encodes a protein with a molecular weight of 43.7KDa, but is named P53 because the protein band appears at 53KDa shown by Marker. Wild-type p53 causes cancer cells to apoptosis, thus preventing canceration; But also has the function of helping cell genes repair defects; Studies have found that mutant p53 can enhance canceration. 50% of human tumors are related to P53 gene. Currently, there are liver cancer, breast cancer, bladder cancer, gastric cancer, colon cancer, prostate cancer, soft tissue sarcoma, ovarian cancer, brain tumor, lymphocyte tumor, esophageal cancer, lung cancer, osteosarcoma, etc. P53 mutation in human tumors is mainly in highly conserved regions, with the highest mutation at 175, 248, 249, 273,…
IL-6: Target Molecule for Cytokine Storms
Interleukin-6 (IL-6) is a multi-effect cytokine that plays an important role in host defense by regulating immune and inflammatory reactions. IL-6 is mainly produced by macrophages, T lymphocytes and B lymphocytes. When the body has inflammatory reaction or infection, various cytokines such as virus, endotoxin and tumor necrosis factor can induce the body to produce IL-6. The biological effects of IL-6 are mainly reflected in inducing B cell differentiation to produce immunoglobulin, promoting T cell proliferation and growth, enhancing blood cell differentiation and its anti-tumor effect, promoting bone marrow hematopoietic stem cell proliferation, etc. IL-6 is an important member of cytokine network and plays a central role in acute inflammatory reaction. The production of IL-6 induces the production of C-reactive…
Research Hotspots of Cell Apoptosis: Bcl-2 Monoclonal Antibody
【Promotion】Abbkine Selected Primary Antibody-Click for Promotion details. Bcl-2 gene (B-cell lymphoma-2) is a member of Bcl-2 family, which is the earliest discovered apoptosis protein. It is an anti-apoptosis protein containing multiple BH domains. It is highly expressed in some cancer cells and selectively plays an anti-tumor role. Bcl-2 family proteins play an important role in the regulation of apoptosis by forming dimers with Bax and dimerizing themselves. When Bcl-2 protein is inhibited, its dimer with Bax decreases, leading to cell apoptosis. When Bcl-2 protein is overexpressed, heterodimers formed by Bcl-2 protein and Bax increase and apoptosis is inhibited. The balance between Bcl-2 and Bax proteins at the cell death signal checkpoint determines the survival or apoptosis of cells. We recommend…
