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IL-6: Target Molecule for Cytokine Storms

Date:2019-10-16 Views:513

Interleukin-6 (IL-6) is a multi-effect cytokine that plays an important role in host defense by regulating immune and inflammatory reactions. IL-6 is mainly produced by macrophages, T lymphocytes and B lymphocytes. When the body has inflammatory reaction or infection, various cytokines such as virus, endotoxin and tumor necrosis factor can induce the body to produce IL-6. The biological effects of IL-6 are mainly reflected in inducing B cell differentiation to produce immunoglobulin, promoting T cell proliferation and growth, enhancing blood cell differentiation and its anti-tumor effect, promoting bone marrow hematopoietic stem cell proliferation, etc.

IL-6 is an important member of cytokine network and plays a central role in acute inflammatory reaction. The production of IL-6 induces the production of C-reactive protein (CRP) and procalcitonin (PCT), which are directly related to inflammatory diseases and infection degree. IL-6 can diagnose early inflammation and warn the occurrence of sepsis faster. In addition, the half-life of IL-6 is shorter than CRP and PCT, which can reflect the effect of antibiotic treatment faster and better reflect the prognosis of patients. At the same time, IL-6 can be used as a biomarker of disease severity and prognosis indicators in cytokine storms, and its expression is superior to TNF-α and IL-1. A large number of research results show that IL-6 is a suitable target molecule for cytokine storms.

If you are studying IL-6 or are about to study IL-6, the recommended IL-6 cytokines are as follows:

Product name #cat Sequence Purity Expressed Host Endotoxin Formulation
Human IL-6 protein PRP100120 Human IL6 (NP_000591.1) (Val 30-Met 212) > 95% E. coli <1EU/μg lyophilized powder
Mouse IL-6 protein PRP100371 Mouse IL6 (P08505) (Phe25-Thr211) > 95% E. coli <1EU/μg lyophilized powder

SDS-PAGE analysis:

Human IL-6 protein
Mouse IL-6 protein

Recommendations for cytokine storage:

Cytokines in lyophilized powder form are very stable and can be stored for several years at -20℃ or -80℃. Freeze-dried powder should be dissolved before use and then added to the culture medium in liquid form. The dissolved cytokines can only be stored for a short time (about 1 week) at 4℃. For long-term preservation, first prepare a diluent (which must contain carrier protein, such as 0.1% BSA, 5%HSA, or 10% FBS), and then sub-pack and freeze at -20℃ or -80℃. Repeated freezing and thawing must be avoided, as each freezing and thawing will cause partial inactivation of proteins.