- Product name
Human IL-6 protein
Amino acid sequence derived from mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 × 105 IU/μg.
- Protein length
The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.3 kDa as estimated in SDS-PAGE under reducing conditions.
- Preparation method
> 95 % as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL6 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Human IL6 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
- Gene ID
- Alternative names
BSF2; HGF; HSF; IFNB2; IL-6; Interleukin-6; Interleukin-6; IL6
Most popular with customers
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Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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