- Product name
Mouse IL-6 protein
Amino acid sequence derived from mouse IL6 (P08505) (Phe25-Thr211) was expressed and purified.
Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 0.4-2 ng/ml. 2. Measured by its ability to bind biotinylated mouse IL6ST-Fc2h (Cat:50135-M03C) in a functional ELISA. 3. Measured by its ability to bind biotinylated mouse IL6ST-His (Cat:50135-M08H) in a functional ELISA. 4. Measured by its ability to bind biotinylated human IL6RB-Fch (Cat:10974-H03H) in a functional ELISA. 5. Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 0.4-2 ng/ml.
- Protein length
The recombinant mouse IL6 consists of 188 amino acids and predicts a molecular mass of 21.9 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
- Preparation method
> 95 % as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL6 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Mouse IL6 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
- Gene ID
- Alternative names
Il-6; Interleukin-6; IL6
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Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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