Human SIGLEC8 ELISA Kit — Application Scenarios for Allergy, Asthma, and Eosinophil Research
SIGLEC8: The Eosinophil's Built-In Brake
Sialic acid-binding Ig-like lectin 8 (SIGLEC8) is predominantly expressed on eosinophils and acts as a brake on these immune cells: its activation induces apoptosis, making it a hot target for asthma, chronic rhinosinusitis, and hypereosinophilic syndromes[reference:19]. Yet most labs still rely on Western blots or polyclonal antibodies that cross-react with SIGLEC5/14, muddying the data[reference:20]. The Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit (KTE60659) from Abbkine is changing how we study eosinophil regulation[reference:21].
Allergy and Asthma Research: From Bench to Clinic
SIGLEC8 is a therapeutic target for eosinophilic asthma. A recent study in the Journal of Allergy and Clinical Immunology used this kit to quantify SIGLEC8 in 100 asthma patient samples, correlating low levels with severe exacerbations (r=0.79, p<0.001)[reference:22]. Allergy researchers use this kit to:
- Quantify SIGLEC8 in serum, plasma, and bronchoalveolar lavage fluid (BALF)
- Monitor eosinophil apoptosis and SIGLEC8 expression dynamics
- Assess the efficacy of anti-SIGLEC8 therapeutics in clinical trials
- Investigate SIGLEC8 as a biomarker for asthma severity and exacerbation risk
The kit detects SIGLEC8 down to 0.039 ng/mL in serum and 0.062 ng/mL in cell culture supernatants[reference:23] — critical for low-input samples like BALF or tear samples from allergic conjunctivitis patients[reference:24].
Chronic Rhinosinusitis and Nasal Polyposis
SIGLEC8 is upregulated in eosinophilic chronic rhinosinusitis. Researchers in this field use this kit to:
- Quantify SIGLEC8 in nasal tissue lysates and nasal swab eluates
- Study the role of SIGLEC8-mediated eosinophil apoptosis in disease resolution
- Investigate SIGLEC8 as a therapeutic target for recalcitrant nasal polyps
Hypereosinophilic Syndromes and Rare Diseases
SIGLEC8 is a critical regulator of eosinophil survival. Researchers studying hypereosinophilic syndromes use this kit to:
- Monitor SIGLEC8 expression in patients with idiopathic hypereosinophilia
- Study the effects of SIGLEC8 agonists on eosinophil apoptosis
- Investigate SIGLEC8 as a pharmacodynamic biomarker in clinical trials
Why Choose This Kit for Your SIGLEC8 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| Ultra-high sensitivity | 0.039 ng/mL in serum; 0.062 ng/mL in supernatants[reference:25] |
| Dynamic range | 0.156–10 ng/mL[reference:26] |
| Exceptional specificity | <1% cross-reactivity with SIGLEC5/14[reference:27] |
| Multiple sample types | Serum, plasma, BALF, nasal swabs, cell culture supernatants[reference:28] |
| Rigorous validation | KO controls, 10+ sample matrices, inter-assay CV <7%[reference:29] |
| Complete components | Ready-to-use — no additional reagents needed |
Kit Components at a Glance
- Human SIGLEC8 microplate (pre-coated with capture antibody)
- Human SIGLEC8 standard
- Human SIGLEC8 detection antibody (biotin-conjugated)
- Streptavidin-HRP
- All buffers and reagents
🔬 Which research field does your work belong to?
Allergy & Asthma Research | Chronic Rhinosinusitis | Hypereosinophilic Syndromes | Drug Development | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit — KTE60659
