Human SFRP2 ELISA Kit — Application Scenarios for Cancer, Fibrosis, and Wnt Signaling Research
SFRP2: The Wnt Decoy You Can Finally Quantify
Secreted Frizzled-Related Protein 2 (SFRP2) is a soluble antagonist of Wnt ligands that modulates cell proliferation, differentiation, and tissue remodeling[reference:30]. Its dysregulation is implicated in cancer, fibrosis, and cardiovascular disease[reference:31]. Yet its low abundance in clinical samples and structural similarity to SFRP1/3 have long complicated accurate quantification[reference:32]. The Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit (KTE60681) from Abbkine offers a rigorously validated solution[reference:33].
Liver Fibrosis Research: A Superior Biomarker
SFRP2 is upregulated in liver fibrosis (up to 8-fold vs. healthy controls) and correlates with disease severity[reference:34]. In a clinical validation, Abbkine tested the kit on 120 liver fibrosis patient samples, correlating SFRP2 levels with Ishak staging (r=0.85, p<0.001) — stronger than traditional markers like hyaluronic acid[reference:35]. Hepatology researchers use this kit to:
- Quantify SFRP2 in serum, plasma, and liver tissue lysates
- Monitor fibrosis progression and treatment response
- Investigate SFRP2 as a non-invasive biomarker for liver fibrosis staging
- Study the Wnt signaling pathway in hepatic stellate cell activation
The kit detects SFRP2 as low as 0.031 ng/mL in serum and 0.049 ng/mL in tissue lysates[reference:36], with a dynamic range of 0.031–6 ng/mL[reference:37].
Cancer Research: Tumor–Stroma Interactions
In pancreatic ductal adenocarcinoma (PDAC), SFRP2 is a marker of desmoplastic stroma[reference:38]. Generic kits have reported false positives in samples with high SFRP1, muddying correlations with patient survival[reference:39]. Oncology researchers use this kit to:
- Quantify SFRP2 in tumor lysates and patient plasma
- Study SFRP2-mediated Wnt pathway dysregulation in cancer
- Investigate SFRP2 as a prognostic biomarker and therapeutic target
- Monitor tumor–stroma interactions and metastatic progression
The kit's dual-epitope strategy — targeting the N-terminal cysteine-rich domain (residues 30–55, <5% homology to SFRP1/3) and the C-terminal netrin-like domain (residues 220–245) — slashes cross-reactivity to <1% with related SFRPs[reference:40].
Cardiovascular Disease and Cardiac Fibrosis
SFRP2 is implicated in cardiac fibrosis and adverse remodeling post-myocardial infarction. Cardiovascular researchers use this kit to:
- Quantify SFRP2 in cardiac tissue lysates and patient serum
- Study Wnt pathway dysregulation in cardiac fibrosis
- Investigate SFRP2 as a biomarker for heart failure risk
Why Choose This Kit for Your SFRP2 Research?
| Feature | Benefit |
|---|---|
| Two-site sandwich ELISA | Quantitative, specific detection |
| Human reactivity | Directly applicable to human samples |
| Ultra-high sensitivity | 0.031 ng/mL in serum; 0.049 ng/mL in tissue[reference:41] |
| Dynamic range | 0.031–6 ng/mL[reference:42] |
| Exceptional specificity | <1% cross-reactivity with SFRP1/3[reference:43] |
| Multiple sample types | Serum, plasma, tissue lysates |
| Superior reproducibility | <6% inter-assay variation in multi-center trials[reference:44] |
| Excellent recovery | 95–103% in spiked samples[reference:45] |
| Complete components | Ready-to-use — priced at $490[reference:46] |
Kit Components at a Glance
- Human SFRP2 microplate (pre-coated with capture antibody)
- Human SFRP2 standard (7-point standard curve)[reference:47]
- Human SFRP2 detection antibody (biotin-conjugated)
- Streptavidin-HRP
- All buffers and reagents
🔬 Which research field does your work belong to?
Liver Fibrosis Research | Cancer Biology | Cardiovascular Research | Wnt Signaling | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!
📌 Reference Link: Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit — KTE60681
