WB|Universal WB Toolkit arranges the WB experiment well
Western Blotting is a powerful technique that allows you to easily detect proteins and estimate their expression and molecular weight. Samples start with a mixture of proteins extracted from cells or tissues. The complete protein western blot assay process consists of several separate steps, each of which is critical to the final data quality. In the actual operation process, the following problems are often encountered:
1.The sample pyrolysis effect is poor, the sample contains more DNA and cell particle impurities interference;
2.Reagent variety, and difficult configuration, after repeated use;
3.Lack of WB positive control, abnormal results can not determine the operation or sample problems;
4.ECL luminescence solution is not sensitive enough to show clear bands of low abundance proteins.
Here we take stock of WB's basic experimental process:
From above, we is not hard to find, based on the traditional the WB experiments of time-consuming, complicated reagent supplies a wide variety, high background, the results of poor stability pain points, Abbkine develop Universal WB Toolkit, the carefully designed study, one-stop operation, can meet the demand of conventional WB experiment, to obtain excellent results.
1. WB experiment is only successful if the sample is preprocessed, and Lysis of the sample is the most important step. Abbkine scientists make continuous efforts to optimize the functions of RIPA and develop Lysis Buffer that also completely dissolves the insoluble components of RIPA.
The protein spectrum obtained by lysis is more complete and the detection result is more reliable.
2. In the process of WB experiment, there are many reagents and consumables involved, and corresponding reagents need to be prepared, including: The preparation of Running Buffer, Transfer Buffer, TBST, BSA blocking solution, etc., includes weighing, dissolving, mixing, PH control and other steps of these reagents. If any error occurs in any detail, it may lead to the failure of WB experiment. These reagents are expensive and uneconomical to buy separately in the world. Abbkine developed a universal western blotting (WB) kit to design and prepare these reagents for WB experiments, eliminating the difficulty and time consuming problem of reagent preparation.
3. WB experiment results are often abnormal or failed due to operation, reagents, instruments and other reasons. When you are at a loss to find out the reason, Abbkine Universal WB Toolkit provides you with ready-to-use WB positive control, which can be directly taken as samples to provide a real WB experiment reference. Help to find out the abnormal interference factors in the experiment;
4. Finally, in order to help customers solve the problem of low protein signal, we also added SuperKine™ SuperKine™ West Femto Maximum Sensitivity Substrate to the kit, which is a highly sensitive color rendering solution to enhance the chemical signal of immunology experiments and obtain excellent experimental results.
The Abbkine Universal Western Blotting (WB) kit, as shown here, is cleverly designed to show:
Kit components Size (20 T) Storage conditions
|Kit components||Size (20 T)||Storage conditions|
|Part 1 of 2||Lysis Buffer||2 mL||4℃|
|Running Buffer (10×)||100 mL||4℃|
|Transfer Buffer (10×)||100 mL||4℃|
|TBST (10×)||100 mL||4℃|
|PVDF Membrane (8.5 cm×6 cm)||2||RT|
|SuperKine™ West Femto Maximum Sensitivity Substrate A||4 mL||4℃, protected from light|
|SuperKine™ West Femto Maximum Sensitivity Substrate B||4 mL||4℃|
|Part 2 of 2||WB Positive Control||40 μL||-20℃|
|Anti-GAPDH Mouse Monoclonal Antibody (2B5)||6 μL||-20℃|
|Colorcode Prestained Protein Marker (10-180 kDa)||5 μL||-20℃|
|HRP, Goat Anti-Mouse IgG||3 μL||-20℃|
|HRP, Goat Anti-Rabbit IgG||3 μL||-20℃|
|SDS-PAGE Loading Buffer (5×)||1 mL||-20℃|
Finally, in order to meet the needs of the vast number of fans, with the continuous efforts of Abbkine r&d experts, we launched a universal western blot (WB) kit, which directly hits the pain points of traditional WB experiments, makes WB experiments simple, efficient and convenient, meets your different experimental needs, and helps your proteomics research career.
Related products are recommended as follows:
|Product NO||Product Name||Application||Size||Price|
|KTD106-EN||Universal WB Toolkit||WB||20T||$59|
|BMU101-EN||SuperKine™ West Pico PLUS Chemiluminescent Substrate||WB||100/500 ml||$59/179|
|BMU102-EN||SuperKine™ West Femto Maximum Sensitivity Substrate||WB||100/500 ml||$69/209|
|BMU103-EN||SuperKine™ Enhanced Antibody Dilution Buffer||WB，IF，ICC，IHC，ELISA||100/500 ml||$59/179|
|ABL1020||Anti-GAPDH Mouse Monoclonal Antibody (2B5)||IF, IHC-P, WB||50/200/200μl*5||$59/109/499|
Abbkine technical experts in order to provide readers with a platform to learn biological knowledge, through unremitting efforts, will be a variety of high-end biological experiments out of dry goods, later published in wechat public number, please love science readers continue to pay attention to Abbkine public number.
Abbkine focuses on proteomics and cytology and is committed to the innovation and development of antibodies, proteins, analytical reagents and kits to become a key enabler of life science research and development, drug discovery and other fields. We provide you with the most popular products for the users of protein and immunology research, from the basic immunology products, such as protein extraction quantification, to internal reference labeled antibodies, primary antibodies and secondary antibodies for immunology experiments. Cell research users' favorite products, from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining tracer and cell metabolism detection products, to cytokines and protein detection kits for cell culture, just to help your research career!