| Product name | SuperKine™ Enhanced Antibody Dilution Buffer |
| Applications notes | SuperKine™ Enhanced Antibody Dilution Buffer provides an enhanced reagent, which can be directly used to dilute the primary or secondary antibody in an appropriate ratio with this product. |
| Alternative | Enhanced Antibody Dilution; Antibody Dilution |
| Kit components | • SuperKine™ Enhanced Antibody Dilution Buffer |
| Features & Benefits | SuperKine™ Enhanced Antibody Dilution Buffer is innovatively optimized based on the classic antibody diluent components to increase the storage time of the diluted antibody and can be used repeatedly. |
| Usage notes | • Do not mix components of different batch numbers and different manufacturers, otherwise it may cause abnormal results. • Try to incubate the antibody at 4°C , which can prolong the life of the antibody after dilution and is also convenient for repeated use. • If non-specific bands interfere with the experiment, you can try to appropriately reduce the amount of protein loaded, reduce the concentration of the primary antibody and the secondary antibody, or shorten the exposure time. • The products have passed strict quality inspections. Welcome to contact us at any time, we are committed to customer success and customer satisfaction. |
| Storage instructions | Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SuperKine™ Enhanced Antibody Dilution Buffer is a ready-to-use universal antibody dilution, suitable for WB, IF, IHC, ELISA and other immunology experiments. By adding professional immune signal enhancement components and uniquely developed immune blocking and stabilizing components, this antibody dilution can be used for various primary or secondary antibodies to enhance the signal intensity of immune experiments and reduce the reaction background. In order to obtain good experimental results. This product does not contain protein, phosphate, sodium azide or thimerosal preservatives, and is suitable for most types of antibodies dilution. |
| Alternative | Enhanced Antibody Dilution; Antibody Dilution |
Fig.1.WB result comparison between BMU103-EN and other brand shows Abbkine SuperKineTM Enhanced Antibody Dilution Buffer detects a more obvious signal. Sample: HEK293 Cells; Primary Antibody: NFkB p65 Monoclonal Antibody (ABM0017, 1:5000).
Fig.2. The picture on the left is the result of IHC verification of SuperKine™ Enhanced Diluent, and the picture on the right is the negative control without primary antibody. The sample is mouse liver tissue. The primary antibody is TNF-α polyclonal antibody (ABP0127, 1:200), and the secondary antibody is goat anti-rabbit IgG (A21020, 1:300).
Fig.3. The left picture is the result of SuperKine™ enhanced dilution IF results, and the right picture is the negative control without primary antibody. The sample is Hela cell. The primary antibody is CK7 Monoclonal Antibody (ABP0127, 1:200), the secondary antibody is Dylight 549, Goat Anti-Mouse IgG (A23310, 1:200).
Fig.4. The picture shows the ELISA verification result of SuperKine™ enhanced diluent, and the standard curve of the KET7011 EliKine™ Mouse IL-12 p70 ELISA Kit after using the diluent.
It can replace PBS, and has the following advantages: improve sensitivity, reduce background, improve antibody stability, widely used.
This product does not contain TritonX-100, if the experimental requirements of cell membrane rupture, you can use triolatone to break the membrane in advance, and then incubate with diluted antibody.
This product contains an appropriate amount of antibody stabilizer, but does not contain preservatives. Currently, it does not have the function of anti-corrosion of antibodies. Attention should be paid to prevent the introduction of microorganisms in the process of use.
Author：J Deng, L Long, X Peng, W Jiang, Y Peng Publication name：European Journal of Medicinal Chemistry IF：6.7
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.