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Human Spondin-1 (SPON1) ELISA Kit

Human Spondin-1 (SPON1) ELISA Kit

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Specification

Product name Human Spondin-1 (SPON1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Spondin-1 (SPON1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPON1 in samples. An antibody specific for SPON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPON1; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein

Product Properties

Kit components • Human Spondin-1 microplate
• Human Spondin-1 standard
• Human Spondin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Spondin-1 (SPON1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPON1. No significant cross-reactivity or interference between Human SPON1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Spondin-1 is a protein contains 807 aminoacids and is structurally composed of six thrombospondin domains, one reelin domain, and one spondin domain.The deduced 624-amino acid partial SPON1 protein shares 96.8% amino acid sequence identity with the rat F-spondin precursor across 624 residues.SPON1 expression in lung, lower expression in brain, heart, kidney, liver, and testis, and lowest expression in pancreas, skeletal muscle, and ovary; no expression was found in spleen. In vitro binding assays using mutated human proteins confirmed that SPON1 specifically bound to the central APP domain (CAPPD). SPON1 inhibited APP cleavage by BACE1, the primary beta-secretase involved in APP processing. Binding also impaired APP- and FE65-dependent transactivation of the chromosome remodeling factor TIP60 .
Gene ID 10418
Alternative SPON1; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein
Accession Q9HCB6

Image & description

Fig.1. Human Spondin-1 (SPON1) Standard Curve.

Fig.1. Human Spondin-1 (SPON1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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