Login Register
English
0

Cart

$ 0

Human Neurobeachin (NBEA) ELISA Kit

Human Neurobeachin (NBEA) ELISA Kit

Views(93) Publications(0) Catalog no(KTE61357)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Neurobeachin (NBEA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Neurobeachin (NBEA) ELISA Kit employs a two-site sandwich ELISA to quantitate NBEA in samples. An antibody specific for NBEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNBEA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NBEA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NBEA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NBEA; RP11-270C18.1; BCL8B; LYST2; lysosomal trafficking regulator 2

Product Properties

Kit components • Human Neurobeachin microplate
• Human Neurobeachin standard
• Human Neurobeachin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Neurobeachin (NBEA) ELISA Kit has high sensitivity and excellent specificity for detection of Human NBEA. No significant cross-reactivity or interference between Human NBEA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NBEA encodes a member of a large, diverse group of A-kinase anchor proteins that target the activity of protein kinase A to specific subcellular sites by binding to its type II regulatory subunits. Brain-specific expression and coat protein-like membrane recruitment of a highly similar protein in mouse suggest an involvement in neuronal post-Golgi membrane traffic. Mutations in this gene may be associated with a form of autism. NBEA and its expression are frequently disrupted in patients with multiple myeloma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants may exist, but their full-length nature has not been determined.
Gene ID 26960
Alternative NBEA; RP11-270C18.1; BCL8B; LYST2; lysosomal trafficking regulator 2
Accession Q8NFP9

Image & description

Fig.1. Human Neurobeachin (NBEA) Standard Curve.

Fig.1. Human Neurobeachin (NBEA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Neurobeachin (NBEA) ELISA Kit”