| Product name | Anti-HA Tag Mouse Monoclonal Antibody (4F6) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Mammals, Bacteria |
| Applications | IF, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB(1:10000-1:100000), IF (1:2000), IP (1:400). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | HA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
| Alternative | HA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain |
Fig.1. Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2.IP (1:400)-WB (1:10000) analysis of HA fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with HA-tag protein (lane B); IP untransfected 293 cell lysate with Anti HA tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D), or with Anti HA tag mAb (lane E), and IP transfected 293 without both normal Mouse IgG and HA tag mAb (lane F).
Author：Huan-Bin Shi, Nan Chen, Xue-Ming Zhu, et al Publication name：Environ Microbiol. 2019 May 30. doi: 10.1111/1462-2920.14699. IF：4.96
Author：Baoquan Han, Kun Xu, et al Publication name：Fish & Shellfish Immunology. 2018; 84: 948-954. IF：3.62
Author：Mengjiao Guo, Rong Li, et al Publication name：Frontiers in Cellular Infection Microbiology. 2018; 8: 220. IF：3.62
Author：Hanbing He, Yougong Peng, et al Publication name：FEBS Letters. 2018; 592(13): 2334-2340. IF：3.43
Author：Baoquan Han, Kun Xu, et al Publication name：Fish & Shellfish Immunology Volume 84, January 2019, Pages 948-954 IF：3.185
Author：Q Li, X Li, B Wu, Y Niu, R Ma, J Xie, A Ali, R Feng Publication name：Virus Research IF：2.934
Author：Ai Lu, Cyrollah Disoma,YuzhengZhou, et al Publication name：Biochemical and Biophysical Research Communications, 13 April 2019 IF：2.62
Author：T Yuan, Y Wang, Y Jin, S Xu, H Zhang, Q Chen, N Li Publication name：biorxiv IF：
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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