|Product name||SuperKine™ West Femto Maximum Sensitivity Substrate|
|Applications notes||SuperKine™ West Femto Maximum Sensitivity Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.|
|Kit components||• ECL HRP Substrate Reagent A
• ECL HRP Substrate Reagent B
|Features & Benefits||The SuperKine™ West Femto Maximum Sensitivity Substrate developed by Abbkine is based on the innovation and optimization of the classic peroxidase substrate system. It is used for enhanced chemiluminescence (ECL) and can directly replace other expensive ECL products without the need to renew Optimize experimental conditions.|
|Usage notes||• Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.
• Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane.
• Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution.
• Drain excess reagent. Cover blot with clear plastic wrap.
• Expose blot to X-ray film.
|Storage instructions||Stable for two years at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The family of ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. The SuperKine™ West Femto Maximum Sensitivity Substrate is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.|
Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-α Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (A21020, 1:10000). The exposure time is 30s.
Under normal conditions, the ECL working solution can be incubated in 5-10 seconds, from incubation to exposure time of less than 30 seconds.
The 100 mL size of the product is composed of Reagent A 50 mL and Reagent B 50 mL, and the 500 mL size is composed of Reagent A 250 mL and Reagent B 250 mL.
Our company verified that the ECL solution has the best effect in the protein content range of 6.25-50 pg.
The phenomenon is caused by the central part of the high concentration of HRP color substrate consumption too fast, the middle part of the substrate consumption after the end of the light, there are two suggestions for the phenomenon: 1. Decrease protein sample loading and concentration of primary and secondary antibodies; 2. Change to ECL color solution with lower sensitivity, such as BMU101-EN.
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