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EliKine™ Human MMP-9 ELISA Kit

EliKine™ Human MMP-9 ELISA Kit

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Specification

Product name EliKine™ Human MMP-9 ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate MMP-9 in samples. An antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP 9; MMP-9; MMP9; Matrix Metalloproteinase 9; GELB; Type V collagenase; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Product Properties

Kit components • Human MMP-9 microplate
• Human MMP-9 standard
• Human MMP-9 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human MMP-9 ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP-9. No significant cross-reactivity or interference between Human MMP-9 and analogues was observed.
Calibration range 31.25 pg/mL- 2000 pg/mL
Limit of detection 16 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Gene ID 4318
Alternative MMP 9; MMP-9; MMP9; Matrix Metalloproteinase 9; GELB; Type V collagenase; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Accession P14780

Image & description

Fig.1. Human MMP-9 Standard Curve.

Fig.1. Human MMP-9 Standard Curve.

Fig.2. EliKine™ Human MMP-9 ELISA Kit is a sandwich ELISA to quantitate MMP9 in samples.

Fig.2. EliKine™ Human MMP-9 ELISA Kit is a sandwich ELISA to quantitate MMP9 in samples.

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