The West Femto Maximum Sensitivity Substrate occasionally has the phenomenon of overexposure during the exposure of high protein abundance. How to deal with it?
The phenomenon is caused by the central part of the high concentration of HRP color substrate consumption too fast, the middle part of the substrate consumption after the end of the light, there are two suggestions for the phenomenon: 1. Decrease protein sample loading and concentration of primary and secondary antibodies; 2. Change to ECL color solution with lower sensitivity, such as BMU101-EN.
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