Featured Cell Cycle Staining Kit from Abbkine scientific
Cell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry. The DNA of mammalian, yeast, plant or bacterial cells can be stained by a variety of DNA binding dyes. As cells progress through the cell cycle, cells in the G1 phase have one set of paired chromosomes and are uniform with respect to DNA content. On the other hand, the amount of DNA begins to double during S phase, so that the amount of DNA is between one and two times the amount in G1. Cells in G2/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes. Abbkine Cell Cycle Staining Kit utilizes a…
Abbkine newly launched TUNEL Apoptosis Detection Kit (Orange Fluorescence)
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. A failure of cells to undergo apoptosis is a common feature of many cancers thus investigation of apoptosis inducing mechanisms is of particular importance for cancer research and requires suitable detection methods. A hallmark of late apoptosis is extensive genomic DNA fragmentation that generates a multitude of DNA double-strand breaks with accessible 3'-hydroxyl (3'-OH) groups. Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay is a method for detecting DNA fragmentation by labeling the 3’-OH termini in the double-strand DNA…
AbFluor™ 488 Annexin V Apoptosis Detection Kit from Abbkine
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells, which can be detected by fluorescence microscopy or flow cytometry. Propidium iodide (PI) is a fluorescent nucleus dye, impermeant to live cells and early apoptotic cells, but stains late apoptotic, necrotic or dead cells with red fluorescence, binding tightly to the nucleic acids in the cell. Once binding with DNA, the…
Abbkine new arrival-Annexin V Apoptosis Detection Kits
Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is…
Abbkine Scientific Newly Launched Agarose/Magnetic beads Conjugated Tag Antibodies
Abbkine Agarose/Magnetic beads Conjugated Tag Antibodies provide a simple and fast method to study tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose. Immunoprecipitation of epitope-tagged proteins can be performed to determine the proteins cellular localization, to study post-translation modifications or to detect interactions between tagged proteins and other proteins. Relying on advanced protein coupling technology and strict quality control specification, Abbkine high affinity Tag Antibody coupled Agarose/Magnetic beads enables immunoprecipitation (IP) of tagged proteins without antibody contamination. The newly portfolio is just as below. Product name Cat# Application Anti-DDDDK Tag Mouse Monoclonal Antibody, Agarose A02010AGB IP Anti-DDDDK Tag Mouse Monoclonal Antibody, Magnetic Beads A02010MGB IP Anti-HA Tag Mouse Monoclonal Antibody, Agarose A02040AGB IP Anti-HA Tag…
SuperLumia ECL Plus HRP Substrate Kit from Abbkine Scientific
Abbkine SuperLumia ECL HRP Substrate Kits are value-priced, classical peroxidase substrates for enhanced chemiluminescence (ECL) that directly replace costlier products without the need to re-optimize conditions. The family of SuperLumia ECL substrates is a series of ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Plus HRP substrate reagent is specially optimized for femtogram amounts of antigen detection on immunoblots. SuperLumia ECL substrates are deeply favored by researchers for exhibiting excellent performance with high sensitivity and low background. The SuperLumia ECL Plus HRP Substrate Kit comprises of Reagents A and B, which are sized equally. The featured product has below benefits The highest sensitive detection are at the femtogram level Same quality and performance with affordable price Extremely…
EliKine™ Rat VEGF ELISA Kit || Abbkine
With an aim to accelerate the life science research worldwide, Abbkine develops a new series of ELISA Kit called EliKine™. This new group of ELISA Kit soon has become star products among Abbkine products. Abbkine injects a large amount of financial and human resources to develop EliKine™ ELISA Kits, and recently Abbkine is pleased to announce the new release of EliKine™ Rat VEGF ELISA Kit. This product will definitely meet the experiment demands of various scientists. Vascular endothelial growth factor (VEGF), also referred to vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be more specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot…
Getting more research benefits from Abbkine's Cell Counting Kit-8 (CCK-8)
Cell Counting Kit-8 (CCK8) is popular with global distributors and end-users since it is developed. Now let's review the product together. Abbkine's Cell Counting Kit-8 uses the reagent WST-8 as its substrate, compared with MTT, MTS or WST-1 tetra-zolium compounds, our featured CCK8 contains below advantages： 1. More convenient with less than 15 mins Method Preparation Procedure CCK-8 - Add reagent - Measure O.D. MTS Thaw reagent Add reagent - Measure O.D. MTT Thaw reagent Add reagent Dissolve MTT Measure O.D. 2. More sensitive than MTT, MTS Incubation: 37 °C, 5% CO2, 2 hours Detection: CCK8 (450 nm), MTS (490 nm), MTT (570 nm) 3. Less toxic and easy following other cell assays If you haven't used Abbkine's CCK8, I highly suggest that you choose a 100…
Welcome the new arrival of EliKine™ Rat IL-17 ELISA Kit
Abbkine has launched various new products recently. With an unique aim to serve life science better, our lab scientists do their utmost to develop new products. EliKine series ELISA Kits have become the most eye-catching one at present. This new group of Kits is especially high in sensitivity, which makes our Kits different from other same level supplier. If you are interested in knowing more about EliKine ELISA Kits, you can download our EliKine ELISA Kits brochures. We all know that Interleukin 17A, also referred to IL-17 or IL-17A, is a pro-inflammatory cytokine. This cytokine is produced by a group of T helper cell known as T helper 17 cell in response to their stimulation with IL-23. IL17 protein exhibits a high homology…
EliKine™ Mouse CCL2 ELISA Kit from Abbkine Scientific
CCL2 gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. Abbkine Scientific debuts the EliKine™ Mouse CCL2 ELISA Kit which is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse…
