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How to choose appropriate Catalase (CAT) Activity Assay Kit?

Catalase (CAT) is an antioxidant enzyme commonly found in almost all living organisms. Its main function is to catalyze the decomposition of hydrogen peroxide into water and oxygen and remove hydrogen peroxide in the body, thus preventing cells from being poisoned by H2O2. It is one of the key enzymes in the biological defense system and provides an antioxidant defense mechanism for the body.

In this article, for CAT Activity Assay Kit, the author chose different brands (Cayman Chemical, Abcam, Biovision) and compared them with Abbkine CheKine™ Catalase (CAT) Activity Assay Kit (Cat#: KTB1040) from several aspects such as assay principle, detection, sample type and size/price.

Summary: For CAT Activity Assay Kit, the brand Abbkine and Cayman Chemical are the similar in Assay Principle. Methanol is taken as a reaction substrate. While Abcam and Biovision take H2O2 as a reaction substrate.

But H2O2 is not only a single substrate of catalase (CAT), other peroxidases in the sample may interfere with the experimental results, and the standard curve error may be caused by instability of hydrogen peroxide. While methanol is the unique substrate of catalase, other peroxidases cannot use methanol as substrate, so interference signals are avoided.

Details of different brands of products are as follows,

BrandAssay PrincipleSample typeSize/Price
AbbkineThis assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate48T/$40
96T/$60
480T/$180
Cayman ChemicalThis assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate96T/$270

480T/$490
AbcamThe catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.Serum, Plasma, Cell Lysate, Tissue Lysate, Urine100T/$415
BiovisionThe catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.Tissue Homogenate, Cell Lysate, erythrocyte lysate100T/$340

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.