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Using Abbkine CCK-8 to analyze cell toxicity

Date:2021-08-16 Views:1985

Cell toxicity is caused by exogenous toxicant which can damage cells, especially when the toxicant can cause cell death and serious organ dysfunction. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.

The short name of Cell Counting Kit-8 is CCK-8 Kit, which is established based on WST-8 reaction and widely used for cell proliferation/Toxicity, drug screening as well as tumor drug sensitivity test experiment, etc... Abbkine CCK-8 Kit provides one ready-to-use CCK-8 solution, it is convenient and fast which will be your prefect choice to replace MTT method.

The reaction mechanism of CCK-8(WST-8)

Figure.1 The reaction mechanism of CCK-8(WST-8)

Picture Source: https://www.creative-bioarray.com/support/colorimetric-cell-viability-assay.htm

Compared with traditional method for Cell Toxicity/Proliferation experiment, CCK-8 has many noteworthy benefits as you can find in below chart clearly.

Detection method



The water solubility of produced formazan Bad (need to add organic solvent to dissolve before detection Good Good Good
Product fomat Powder   two solutions Solution one CCK-8 solution
Using method Prepare

before use


before use

Ready to use Ready to use
Detection sensitivity High Very High Very High High
Detection time Relatively longer Relatively shorter Relatively Shorter Shortest
Detection wave length 560-600nm 420-480nm 420-480nm 430-490nm
Cytotoxicity High Very Low Very Low Very low
Reagent stability Normal Relatively poor Normal Very good
Batch sample testing Suitable Very suitable Very suitable Very suitable
Convenience degree Normal Convenient Very convenient Very convenient

Apart from above mentioned features, Abbkine made several improvements on the formula of its Cell Counting Kit-8, so I believe you can have a pleasant experience on the use of Abbkine CCK-8 Kit including below points:

◎Less toxic, compatible for other downstream assays

◎No organic solvents or isotopes required

◎No harvesting, no washing and no solubilization steps

◎Low toxicity, the same cells can be used for other cell assays

The typical curve

Fig.2:The typical curve

Insisting in providing high quality and affordable product solution for worldwide scientists, Abbkine deducts almost half of list price than other world renewed CCK-8 Providers. You can see our product specification as below:

Catalogue Number Product Name Size Price
KTA1020 Cell Counting Kit-8 (CCK-8) 200T/1000T/10000T $19/$69/$529

For the first time purchaser, we can provide sample pack for your pre-experiment use.

Abbkine has developed various cell status check tools for scientific researchers in the field of cytology in past three years. From cell proliferation, cell toxicity to cell apoptosis, you can always find one product suitable for your research project. For relative product information, you can check as below:

Research Field Catalogue Number Product Name
Cell Toxicity KTA1030 LDH Cytotoxicity Assay Kit
Cell Senescence KTA3030 Senescence β-Galactosidase Staining Kit
Cell Apoptosis KTA2010 TUNEL Apoptosis Detection Kit (Green Fluorescence)
Cell Apoptosis KTA0002 Annexin V-AbFluor™ 488 Apoptosis Detection Kit
Cell Proliferation KTA2031 Cell Proliferation EdU Image Kit (Orange Fluorescence) C
Cell Apoptosis KTA3022 Caspase-3 Assay Kit (Colorimetric)
Cell Apoptosis KTA3026 Caspase-9 Assay Kit (Colorimetric)


Inspire your cell and protein

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !