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Abbkine mitochondrial respiratory chain complex series detection kits and tricarboxylic acid cycle series detection kits

Date:2021-07-19 Views:449

Some time ago, Abbkine launched a lot of rare treasures with full horsepower, and quickly captured the hearts of a large number of heroes and knights on the rivers and lakes, and received rave reviews. To this end, the editor deliberately shows the members who have recently debuted in the center of the traffic.

Type Item No.  Product Name
Flow Explosion Series BMU101-EN SuperKine™ West Pico PLUS Chemiluminescent Substrate
BMU102-EN SuperKine™ West Femto Maximum Sensitivity Substrate
BMU103-EN SuperKine™ Enhanced Antibody Dilution Buffer
KTD101-EN Universal Loading Control Antibody Cocktail
Mitochondrial respiratory chain complex series KTB1850 CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric)
KTB1860 CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric)
KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric)
KTB1880 CheKine™ Mitochondrial complex Ⅳ Activity Assay Kit (colorimetric)
KTB1890 CheKine™ Mitochondrial complex Ⅴ Activity Assay Kit (colorimetric)
Star single product KTB1060 CheKine™ Advanced Oxidation Protein Products (AOPP) Assay Kit (Colorimetric)
KTB1910 CheKine™ Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric)
Tricarboxylic acid cycle series KTB1023 CheKine™ Citrate Synthase (CS) Activity Assay Kit( Colorimetric )
KTB1230 CheKine™ Succinate Dehydrogenase(SDH)Activity Assay Kit (Colorimetric )
KTB1270 CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Colorimetric)
KTB1240 CheKine™ α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit (Colorimetric )
KTB1250 CheKine™ Mitochondrial Isocitrate Dehydrogenase(ICDHm)Assay Kit (Colorimetric )
KTB1260 CheKine™ Acetyl Coenzyme A (Acetyl-CoA) Assay Kit (Colorimetric )

To say that these popular kings are among the most cost-effective enhanced/hypersensitive ECL luminescent fluids, enhanced antibody diluents, and unique universal internal reference antibody cocktail sets in the flow-exploded series; star single product Advanced Oxidized Protein Products (AOPP) and Reactive Oxygen Species (ROS) kits are born with their own halo properties and are attracting attention; the perfect combination, the tricarboxylic acid cycle series and the mitochondrial respiratory chain complex series are also extraordinary and their advantages are full. In this issue, I will share with you the perfect combination of the mitochondrial respiratory chain complex series and the tricarboxylic acid cycle series.

 

Mitochondrial respiratory chain complex series

Series product testing principles and applicable sample types

Item No. Product Name Assay principle, applicable sample type
KTB1850 CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I. It can be used to determine animal, plant tissue and cell samples.
KTB1860 CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) The reduced coenzyme Q, which is the catalytic product of the mitochondrial respiratory chain complex II, can further reduce 2,6-dichloroindoxyl, which has a characteristic absorption peak at 605 nm. So, the activity of complex II could be calculated according to the decrease rate of 2,6-dichloroindoxyl.
KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) Complex III could transfer the hydrogen from reduced CoQ to cytochrome C to produce reduced cytochrome C. Unlike oxidized cytochrome C, reduced cytochrome C has characteristic light absorption at 550 nm. So, the increasing of light absorption rate can reflect the activity of Complex III enzymes, and Abbkine CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) can detect animal and plant tissues and cell samples.
KTB1880 CheKine™ Mitochondrial complex Ⅳ Activity Assay Kit (colorimetric) Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV. It can be used to determine animal, plant tissue and cell samples.
KTB1890 CheKine™ Mitochondrial complex Ⅴ Activity Assay Kit (colorimetric) Abbkine CheKine™ Mitochondrial complexⅤActivity Detection Kit (colorimetric ) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex Ⅴ hydrolyzes ATP to produce ADP and Pi. The activity of complex Ⅴ is determined by measuring Pi increase rate. It can be used to determine animal, plant tissue and cell samples.

Series product advantages and features

  1. Detected by micro method

Compared with the spectrophotometer method, the micro method makes the experiment operation easier and faster, saves time, and is suitable for multi-sample detection.

  1. Provide detailed and reliable results calculation method

Demonstrate clear and detailed results calculation methods to make experimental results more stable and reliable

  1. Provide a suitable method for protein concentration determination

Each product provides the best method for determining the protein concentration of the sample according to the actual situation

  1. Provide true and reliable sample verification results and references

Experimental result data display

Complex I-II

Complex III-IV

Pi-Complex V

Tricarboxylic acid cycle series

Background introduction

The tricarboxylic acid cycle (tricarboxylic acid cycle) is a cyclic reaction system composed of a series of enzymatic reactions. In the reaction process, firstly, acetyl-Coenzyme A (C2) and oxaloacetic acid (OAA) (C4) are condensed to produce Citric acid with 3 carboxyl groups (C6), after 4 dehydrogenation (3 molecules of NADH+H+ and 1 molecule of FADH2), 1 time of substrate level phosphorylation, finally 2 molecules of CO2 are generated, and oxaloacetic acid is regenerated. process. The tricarboxylic acid cycle is the most effective way for the body to oxidize sugars or other substances to obtain energy; the tricarboxylic acid cycle is the hub of the metabolism, connection and transformation of sugar, lipids, proteins, and even nucleic acids.

Tricarboxylic acid cycle

Series product testing principles and applicable sample types

Item NO. Product Name applicable sample type Assay principle
KTB1023 CheKine™ Citrate Synthase (CS) Activity Assay Kit( Colorimetric ) Animal and plant tissue and cell samples CheKine™ Citrate Synthase (CS) Activity Assay Kit provides a simple, sensitive and rapid CS activity detection method, which is compatible to various biological samples, especially tissues or cell cultures (adherent or suspension) samples of animal and plant. CS activity is determined depending on that CS can catalyze acetyl CoA and oxamic acid to produce citamoyl co-enzyme A, further hydrolysis to produce citric acid; this reaction makes colorless DTNB transform into yellow TNB, TNB has a characteristic absorbing value at 412 nm. The activity of citrate synthase can be obtained by calculating the increase rate of the light absorption at 412 nm.
KTB1230 CheKine™ Succinate Dehydrogenase(SDH)Activity Assay Kit (Colorimetric ) Samples of animal tissues, plant tissues, cells and fungi CheKine ™ Succinate Dehydrogenase (SDH) Activity Assay Kit provides a simple, sensitive and rapid SDH activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension), and fungi. The detection principle is based on that SDH can catalyze succinate to dehydrogenate and produce fumaric acid. The hydrogen can be transmited by Phenazine dimethyl ester sulphuric acid (PMS), thus reducing 2,6-Dichlorophenolindophenol (DCPIP) and 2,6-DCPIP has a special absorption peak at 605 nm. The reducing speed of 2,6-DCPIP can be determined by detecting the change of absorbance at 605 nm, then SDH activity in samples can be calculated.
KTB1270 CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit (Colorimetric) Animal and plant tissue and cell samples Abbkine CheKine™ Pyruvate Dehydrogenase (PDH) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PDH activity detection method, which is compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension). The detection principle is that PDH can catalyze pyruvate dehydrogenation, and reduce 2,6-Dichlorophenolindophenol (2,6-DCPIP), the changes of 2,6-dichlorohenol could be detected by reading the absorption at 605 nm. The enzyme activity of PHD could be obtained by calculating the reduction rate of 2,6-Dichlorophenolindophenol.
KTB1240 CheKine™ α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit (Colorimetric ) Samples of animal and plant tissues, cells and fungi CheKine™ α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit provides a simple, sensitive and rapid α-KGDH activity detection method, which is compatible to various biological samples, especially animals or plant tissues, cell cultures (adherent or suspension) and fungi. The detection principle is based on that α-KGDH can catalyze α-Ketoglutarate, NAD+ and coenzyme A to produce succinyl-coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. α-KGDH activity can be calculated according to the generation rate of NADH.
KTB1250 CheKine™ Mitochondrial Isocitrate Dehydrogenase(ICDHm)Assay Kit (Colorimetric ) Samples of animal and plant tissues, cells and fungi CheKine™ Mitochondrial Isocitrate Dehydrogenase(ICDHm) Assay Kit provides a simple, convenient, and rapid method for detecting isocitrate dehydrogenase activity, which is Suitable for the detection of animal tissues, plant tissues, cells, (adherent or suspension) and fungi. The principle is that ICDHM can catalyze NADP + reduction to produce NADPH, NADPH has an absorption peak at 340 nm, and ICDHM activity can be calculated by the change of light absorption at 340 nm.
KTB1260 CheKine™ Acetyl Coenzyme A (Acetyl-CoA) Assay Kit (Colorimetric ) Samples of animal and plant tissues, cells and fungi CheKine™ Acetyl Coenzyme A (Acetyl-CoA) Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, Suitable for various types of samples, especially animals or plant tissues or cell cultures (adherent or suspension). The detection principle is that malate dehydrogenase can catalyze malate acid and NAD to produce oxaloacetic acid and NADH. Citrate synthase can catalyze Acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, Acetyl-CoA content is proportional to the generating rate of NADH. NADH has a special absorption peak at 340 nm, and the calculation of the absorbance value of 340 nm can be obtained the content of Acetyl-CoA.

Experimental result data display

Citrate Synthase CSPyruvate dehydrogenase

Succinate dehydrogenaseα-ketoglutarate dehydrogenase (α-KGDH)

Mitochondrial isocitrate dehydrogenaseAcetyl Coenzyme A

 

 

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