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New products are coming!

Date:2021-09-07 Views:162

Two new products were launched, including an apoptosis detection kit and a biochemical detection kit. The details are as follows:

Product Name Catalog Number Size
Apoptosis Assay Cocktail KTD102-EN 1 Kit
CheKine™ Amino Acid (AA) Colorimetric Assay Kit KTB1460 96 T

Part 1 Apoptosis Assay Cocktail

Apoptotic cells produces a number of important changes, including cell shrinkage, cytoplasmic agglutination, DNA disruption, and formation of membrane vesicles, and lead to the separation of cell components to form apoptotic bodies, which are eventually phagocytic by macrophages or parenchyma cells[1]. When detect the apoptosis, do you hesitate to choose the methods or experimental principle? Abbkine has the answer!

Abbkine has developed a cocktail set to detect different stages of early and late apoptosis simultaneously based on the new generation of AbFluor™ fluorescent staining and high efficiency recombinant protein technology. Vermes was the first researcher to use Annexin V staining to specifically identify phosphatidylserine (PS) on the ectropion cell membrane, and the Annexin V assay is considered the most classical method by researchers worldwide[2]. In addition, DNA fragmentation is an important characteristic in the late stage of apoptosis, and TUNEL method based on DNA fragmentation detection is regarded as another gold standard for apoptosis research[3]. It’s suitable for different samples such as tissues and cells. High sensitivity and universal apoptosis detection kit can effectively distinguish apoptosis from necrosis. Meanwhile, aiming at the non-standardization of apoptosis detection and results, we developed a patent positive control substance for apoptosis detection, which was combined into the Apoptosis Assay Cocktail. This product can be used as a standardized product for cell apoptosis research and can meet the needs of most cell apoptosis detection. It is the best choice for cell apoptosis detection. Abbkine has developed a highly sensitive, universal apoptosis kit that can detect early stage and late stage apoptosis simultaneously, and it’s suitable for both tissues and cells, and effectively distinguish between apoptosis and necrosis. This product can be used as a standardized product for cell apoptosis research and can meet the needs of most cell apoptosis detection. It is the best choice for cell apoptosis detection.

Component name 规格
Annexin V-AbFluor™ 488 Apoptosis Detection Kit 40 T
TUNEL Apoptosis Detection Kit (Green Fluorescence) 20 T
Apoptosis Positive Control 5 T

Advantages

1 A standardized kit for apoptosis studies.

Annexin V assay and TUNEL assay, as the two gold standards for apoptosis detection, almost cover most of the requirements for apoptosis detection. The unique Apoptosis Assay Cocktail not only includes the above two detection methods, but also a patented positive control for apoptosis, which perfectly solves the problem of non-standardization of apoptosis detection and results.

2 High sensitivity, universal apoptosis detection kit.

After optimization, the kit can simultaneously detect different stages of early stage and late stage apoptosis, suitable for different samples such as tissues and cells, and effectively distinguish apoptosis from necrosis.

3 Widely used.

It can be used for both flow cytometry and fluorescence microscopic observation.

Results

HeLa cells were induced by inducers A and B as positive control group, and normal cells as negative control. Apoptosis detection cocktail kit was used for detection. Annexin V-Abfluor ™ 488 has a high affinity for PS, and PS exposed through the outer side of the cell binds to the membrane of cells in the early apoptotic stage, which were marked fluorescent green and the nucleus were marked red fluorescent by PI after structural damage.

Fig.1 Annexin V-AbFluor™ 488 Apoptosis Detection Kit was used to detect the apoptosis effect of HeLa cells after apoptosis induction

After Annexin V-Abfluor ™ 488 labeling, the necrotic cells and apoptotic cells in early stage and late stage were accurately identified.

Fig.2 Flow cytometry was used to detect the apoptosis rate by different concentrations of inducer A and B.

In the detection of apoptosis by TUNEL assay, deoxyribonucleotide terminal transferase (TdT) was used to label the deoxyribonucleotide - luciferase conjugates to the 3'- terminus of the DNA gap, and apoptosis was visualized.

Fig.3 TUNEL Apoptosis Detection Kit (Green Fluorescence) was used to detect the apoptosis effect of HeLa cells after apoptosis induction.

Part 2 CheKine™ Amino Acid (AA) Colorimetric Assay Kit

CheKine™ Amino Acid (AA) Colorimetric Assay Kit, works by combining the α-amino of amino acids with hydrated ninhydrin to produce a blue-purple compound with characteristic absorption peaks at 570 nm. Then amino acid content can be calculated by measuring absorbance.

Advantages

1 Determination of amino acids in serum, plasma, tissue/cell samples, urine and other biological fluids;

2 Detection range: 0.625-40 μmol/mL;

3 Simple operation steps;

4 Detailed sample preparation and result calculation methods are provided.

Fig.4 Standard curves for determination of amino acid content by CheKine™ Amino Acid (AA) Colorimetric Assay Kit

Fig. 5 The amino acid content of different sample species was detected by CheKine™ Amino Acid (AA) Colorimetric Assay Kit

According to Fig. 4 and Fig. 5, the CheKine™ Amino Acid (AA) Colorimetric Assay Kit was used to obtain perfect standard curves, which were verified in both animal tissues (liver, spleen, lung of mice) and plant tissues (aloe, cynoglossa, and flesh-eating plants) to obtain accurate amino acid contents.

For more product information please pay attention to Abbkine official website! Welcome to order!

References

[1] Palanivel K, Kanimozhi V, Kadalmani B , et al. Verrucarin A induces apoptosis through ROS-mediated EGFR/MAPK/Akt signaling pathways in MDA-MB-231 breast cancer cells.[J]. J Cell Biochem, 2015, 115(11):2022-2032.

[2] Vermes I, Haanen C, Steffens-Nakken H et al. A novel assay for apoptosis.Flow cytometric detection of phosphatidylserine expression on early apoptosis cells using fluorescein labeled Annexin V. J Immunol Methods,1995; 184:39.

[3] Gorczyca W, Gong J P, Darzynkiewicz Z.Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assay. Cancer Res,1993;53:1945.

 

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