Immunofluorescence Master Key - Universal IF Toolkit
We usually encounter many difficult problems when doing immunofluorescence experiments, such as weak staining or no signal, high background and strong non-specific signal, and for example, the hard work of slices, which was discovered only during the final observation. And for example, all the reagents for immunofluorescence are self-prepared, and the configuration time is also real. So how to solve these very common problems? Let's take a look.
Immunofluorescence technology (IF) is based on the principle of antigen-antibody reaction. First, a known antigen or antibody is labeled with fluorescein to make a fluorescent antibody, and then the fluorescent antibody (or antigen) is used as a probe to detect tissue or cells. the corresponding antigen (or antibody).
Based on the tedious and time-consuming operation of traditional IF experiments, a wide variety of reagents and supplies, and poor stability and reliability of experimental results, Abbkine has developed a Universal IF Toolkit.
Advantages of Universal IF Toolkit:
①The operation is convenient, the components are complete, and there is no need to worry about complex reagents, and the experiment can be started immediately after opening the box;
②Provide optimized antibody diluent and SuperKine™ Enhanced Antifade Mounting Medium, which has strong anti-quenching effect and maintains excellent fluorescence intensity;
③Provide Dylight series secondary antibodies with stronger fluorescence intensity and higher specificity;
④Provide high-quality goat serum for blocking to reduce background interference;
⑤Provide nuclear staining, DAPI (500X), for easy nuclear localization.
- Immunostaining Permeabilization Buffer
- EDTA Antigen Retrieval Solution pH 8.0 (20×)
- PBS (20×)
- Goat Serum Blocking Buffer
- Antibody Wash Buffer (20×)
- Antibody Dilution Buffer
- DyLight 488, Goat Anti-Rabbit IgG/DyLight 488, Goat Anti-MouseIgG/DyLight 594, Goat Anti-Rabbit IgG/DyLight 594, Goat Anti-MouseIgG（One secondary antibody per IF Toolkit）
- DAPI (500×)
- SuperKine™ Enhanced Antifade Mounting Medium
Universal IF Toolkit is a multifunctional kit developed by Abbkine for immunofluorescence
staining. It is very convenient and practical, and can quickly carry out IF experiments and obtain high-definition colorful imaging pictures.
|Catalog No||Product Name||Size||Price/$|
|KTD107-EN||Universal IF Toolkit (Anti-Rabbit Dylight 488)||100T||139|
|KTD108-EN||Universal IF Toolkit (Anti-Mouse Dylight 488)||100T||139|
|KTD109-EN||Universal IF Toolkit (Anti-Rabbit Dylight 594)||100T||139|
|KTD110-EN||Universal IF Toolkit (Anti-Mouse Dylight 594)||100T||139|