New arrival |【Annexin V/ANXA5】Recombinant Human Annexin A5

【Annexin V/ANXA5】Recombinant Human Annexin A5

Introduction to Annexin V

Annexin V is a calcium ion-dependent phospholipid binding protein with a molecular weight of 35~36KDa, which can specifically bind to phosphatidylserine PS with high affinity. In normal cells, phosphatidylserine is only distributed on the inner side of the lipid bilayer of the cell membrane. In the early stage of cell apoptosis, the membrane phosphatidylserine (PS) turns from the inner side of the lipid membrane to the outer side. As a phospholipid binding protein, Annexin V has a high affinity with phosphatidylserine. It binds to the cell membrane of cells in the early stage of apoptosis through the phosphatidylserine exposed on the outside of the cell. Therefore, Annexin V is a sensitive indicator for detecting early cell apoptosis.

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Demonstration of the experiment results

Fig. Abbkine lyophilized annexin V protein, lyophilized at 37°C for 4 days, 37°C for 7 days, and A brand annexin V protein, are respectively  labeled  with AbFluor488 fluorescent dye for apoptosis staining using LinKine™ AbFluor™ 488 Labeling Kit (abbkine, KTL0520-10k) . Hela cells were treated with Biyuntian Cell Apoptosis Positive Control Kit (C0005, 1:1000 dilution) for 24 hours, then stained and observed by fluorescence microscope.

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Advantages of Abbkine recombinant protein products

a：Provide reconstitution buffer

Each tube of lyophilized powder product is equipped with a reconstitution buffer (1mL), which makes the experiment operation simple and fast.

b：High purity

Detected by SDS-PAGE, the purity is above 95%.

c：Low endotoxin

Measured by the LAL method, the endotoxin content per microgram of protein is <1 EU.

d：Short delivery time

Domestic shipping time does not exceed 3 days.

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