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Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT)

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Specification

Product name Terminal Deoxynucleotidyl Transferase (TdT)
Sequence Amino acid sequence derived from bovine TdT (P06526) (Mer1-Ala509) was expressed.
Protein length The recombinant bovine TdT consists of 509 amino acids and migrates with an apparent molecular mass of 58.3 kDa as estimated in SDS-PAGE under reducing conditions.
Purity > 90% as determined by SDS-PAGE
Alternative DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase

Product Properties

Formulation The recombinant bovine TdT reconstitution solution, the compound solution is a sterile aqueous solution, 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% BSA, 50% Glycerol, pH 6.5.
Kit components • Recombinant bovine TdT reconstitution solution
•5X Reaction Buffer
Molecular weight 58.3 kDa
Usage notes Please centrifuge before opening the lid. This product is a protein complex solution, and the concentration is not less than 2 mg/ml. Customers can make further dilutions according to subsequent experimental needs.
Storage buffer Liquid in sterile 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% BSA, 50% Glycerol, pH 6.5.
Storage instructions Stored at -20°C.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Terminal deoxynucleotidyl transferase (TdT), also known as terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. Generally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases, it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends.
Gene ID 9913
Alternative DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Accession P06526

Image & description

Fig. SDS-PAGE analysis of TdT.

Fig. SDS-PAGE analysis of TdT.

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