Annexin V-AbFluor™ 555 Apoptosis Detection Kit

Product Information

  • Product name

    Annexin V-AbFluor™ 555 Apoptosis Detection Kit

  • Application notes

    Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit contains Annexin V labeled with our proprietary orange fluorescent dye AbFluor™ 555, which allows the identification and quantification of apoptotic cells by flow cytometry or fluorescence microscopy.

Fig. Annexin V-AbFluor™ 555 Apoptosis Detection Kit allows the identification and quantification of apoptotic cells by flow cytometry or fluorescence microscopy.

Product Properties

  • Kit components

    • Annexin V Binding Buffer (5x) • Annexin V- AbFluor™ 555

  • Features & Benefits

    • Abbkine AbFluor™ 555 dye provides the same or better fluorescence and photostability as Alexa Fluor® 555, Cy™3, TRITC • Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm • Suitable for flow cytometry or fluorescence microscopy

  • Storage instructions

    Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze.

  • Shipping

    Blue ice

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet.

  • Alternative names

    Annexin 5; Annexin V; AbFluor™ 555

Most popular with customers

Reviews

There are no reviews yet.

Be the first to review “Annexin V-AbFluor™ 555 Apoptosis Detection Kit”

Your email address will not be published. Required fields are marked *

Annexin V-AbFluor™ 555 Apoptosis Detection Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTA0003

  • Size
  • Price
  • 20T $60
  • 50T $120
  • 100T $200

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

Gaining Your Trust with our

High quality *
Attractive price
100% Guarantee
Secured shipment