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What is the significance of IgG-negative controls in the IP/ co-IP experiment?

Date:2022-03-10 Views:357

Rule out the possibility of non-specific binding, such as testing a rabbit cell for a protein, if there is no IgG control and the operation has a non-specific protein that interacts with the designed antibody, the result will be positive. If lgG negative control is done, the control does not appear positive, indicating that there is no non-specific protein, the control appears positive, indicating that the antibody has the possibility of non-specific binding to the hybrid protein, need to start the experiment again.


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