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How to quickly, accurately and stably label the subcellular structure of living cells and fixed cells

TraKine™ Pro is a series of long-term super-resolution cell staining imaging combination, used to mark the subcellular structure of living cells and fixed cells, through the design sequence to achieve specific binding with the target Marker protein gene sequence, and with high brightness and The resolution of the fluorescence is directly coupled. The unique fluorescent dyes of the TraKine™ Pro series cover the spectrum from ultraviolet to near-infrared. This time I emphatically recommend the TraKine™ Pro series of selected popular research targets, which occupy a place in the scientific research field, including: KTC4100-1                                                       …

Cytokines, what you need and what we have

Cytokines are small proteins (~5–20 kDa) that are crucial in controlling the growth and activity of other immune system cells and blood cells. Cytokines have been shown to be involved in autocrine, paracrine and endocrine signaling as immunomodulating agents. When released, they signal the immune system to do its job.   (Image source: Google) Cytokine is a general name; other names include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced…

How to draw ELISA standard curve with CurveExpert 1.4 Software

The standard curve of ELISA generally uses special curve fitting tools, such as: CurveExert 1.4. CurveExpert 1.4 is a comprehensive curve fitting system for Windows. XY data can be modelled using a toolbox of linear regression models, nonlinear regression models, interpolation, or splines. Next, we will take Curve Exert1.4 as an example to briefly introduce how to make the standard curve of ELISA: Start "Curve Expert 1.3" software. Enter the OD value of the standard on the X axis and the corresponding concentration value on the Y axis, as shown below: Click the [Run] button (red circle above), and the following dialog box appears:      4. Click the [0K] button, the following two dialog boxes appear, and close the…

Abbkine Year-End big sale: Register to get extra discount and free gift!

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Using Purekine "fishing gear" to fish, the protein club "will be on the bait"

In the last issue, the editor brought you "Dry Goods|Fishing with IP "Hooks", Protein Club "Wishers Take the Bite", and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting "fishing" activity, then the Purekine filler we use is a tough and slender "fishing rod" and "fishing line". The known GST-labeled recombinant protein is sharp And the perfect combination of strong "fish hook" and "fish bait", and our target protein is the "fish" that moves freely in the water. The concept of Pull-Down is similar to Co-IP, and its purpose is to study…

Role and function of IL-1 / IL-4 / IL-6 / IL-33

Interleukins (ILs) are a group of cytokines secreted by macrophages, lymphocytes and other cells, and were first seen to be expressed by white blood cells (leukocytes). According to different structural characteristics, interleukin can be divided into four categories. However, their amino acid sequence similarity is rather weak (typically 15–25% identity). The main biological functions of the Interleukins are involved in immune response and immune regulation, participating in the molecular system of intercellular signal transmission composed of nerve, endocrine system and cytokines, and involved in inflammation and immunopathological tissue damage. Different interleukins have different sources and functions. Some interleukins can be produced by a variety of immune cells, and one interleukin can also play a role in a variety of cells.…

Using immunoprecipitation "fish hooks" for fishing, the protein will always "hooked with willing"

The thoughtful Thursday is here again, and the editor who has long been unable to bear it is finally released to meet our favorite "Abbkine Fans". This time, the editor brings you a wave of colorful biological science mysteries-protein interaction. If protein interaction is compared to an interesting "fishing" activity, then the tag antibody coupling carrier we use is a sharp and strong "fish hook", and the tag recombinant protein (bait protein) is sweet and delicious. "Bait", and our target protein is the "fish" that moves freely in the water. If you want to do your job well, you must first sharpen your tools. Maybe you are still worrying about the purification and enrichment of the target protein without a…