KTE100947 | Rat Estradiol antibody (E2-Ab) ELISA Kit — Quantify the Autoantibody That Reflects Estrogen Status

E2-Ab: The Autoantibody That Tracks Estrogen Exposure

Estradiol (E2) is the most potent endogenous estrogen, essential for female reproductive function and present at lower levels in males. But estradiol is not only a hormone—it can also act as a hapten, triggering the production of anti-estradiol antibodies (E2-Ab). Quantifying E2-Ab levels provides insight into estrogen exposure, endocrine disruption, and possibly autoimmune responses. In rat models, E2-Ab measurement is valuable for reproductive toxicology, endocrine research, and immunology studies.

Competitive ELISA: Designed for Small-Molecule Antibody Detection

KTE100947 employs the competitive enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to E2-Ab. Standards or samples are added alongside HRP-conjugated E2-Ab, and a competitive inhibition reaction occurs between HRP-labeled and unlabeled E2-Ab for antibody binding sites[reference:25]. Color develops inversely proportional to E2-Ab concentration[reference:26].

Rat-Specific, Endocrine-Immunology Research Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:27]. No significant cross-reactivity or interference between Rat E2-Ab and analogues was observed[reference:28].

Specifications

• Assay Type: Competitive ELISA (quantitative)[reference:29]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:30]
• Assay Duration: 3–5 hours[reference:31]
• Storage: 2–8°C unopened[reference:32]
• Shipping: Gel pack with blue ice[reference:33]

Complete Kit Components

The kit includes: Rat E2-Ab microplate, Rat E2-Ab standards, Rat E2-Ab detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:34].

Who Needs This Kit?

• Reproductive toxicologists studying estrogen exposure and endocrine disruption
• Endocrinologists investigating estrogen metabolism and immune responses
• Immunologists studying hormone-autoantibody interactions
• Pharmacologists evaluating estrogen-based therapeutics
• Environmental toxicologists assessing xenoestrogen exposure

🔗 Reference Link: https://www.abbkine.com/product/rat-estradiol-antibody-e2-ab-elisa-kit-kte100947/