KTE100838 | Rat Glutathione (GSH) ELISA Kit — The Master Antioxidant, Quantified with Precision

GSH: The Cell's First Line of Defense Against Oxidative Stress

Glutathione (GSH) is the most abundant intracellular thiol and the primary antioxidant defense system in mammalian cells. This tripeptide (γ-glutamyl-cysteinyl-glycine) directly neutralizes reactive oxygen species, regenerates other antioxidants (vitamins C and E), and maintains the redox balance essential for cellular function. GSH depletion is a hallmark of oxidative stress and is implicated in aging, neurodegeneration, liver disease, and cancer. In rat models, GSH quantification is a critical readout for oxidative stress assessment.

Competitive ELISA: Accurate Quantification of a Small-Molecule Antioxidant

As a small molecule, GSH requires a competitive assay format for accurate quantification. KTE100838 employs the competitive enzyme immunoassay technique, where sample GSH competes with HRP-conjugated GSH for binding to a pre-coated antibody. The intensity of color development is inversely proportional to GSH concentration—the more GSH in your sample, the weaker the signal.

Rat-Specific, Antioxidant-Research Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, tissue homogenates, and cell culture supernatants. Whether you're studying hepatic oxidative stress, cardiac ischemia-reperfusion, or neuroprotection, KTE100838 delivers GSH data you can trust.

Specifications

• Assay Type: Competitive ELISA (quantitative)
• Sample Types: Serum, Plasma, Tissue homogenates, Cell culture supernatants
• Assay Duration: 3–5 hours
• Storage: 2–8°C unopened
• Shipping: Gel pack with blue ice

Complete Kit Components

The kit includes: GSH microplate, GSH standards, HRP-conjugated GSH, detection antibody, substrate solutions, stop solution, wash buffer, and plate covers.

Who Needs This Kit?

• Toxicologists assessing compound-induced oxidative stress
• Cardiovascular researchers studying ischemia-reperfusion injury
• Neuroscientists investigating neurodegeneration and neuroprotection
• Hepatologists evaluating liver injury and fibrosis
• Aging researchers studying redox balance in senescence

🔗 Reference Link: https://www.abbkine.com/product/rat-glutathione-gsh-elisa-kit-kte100838/