KTE101019 | Rat Cardiac troponin I (cTn-I) ELISA Kit — The Most Specific Biomarker for Cardiac Injury, Now for Rat Models

cTn-I: The Unmistakable Signature of Myocardial Damage

Cardiac troponin I (cTn-I) is one of three subunits of the troponin complex in cardiac muscle—alongside troponin T (TnT) and troponin C (TnC)[reference:35]. As the inhibitory subunit, TnI prevents actin-myosin interaction in the absence of calcium. But more importantly, cTn-I is exclusively expressed in cardiac muscle—making it the most specific biomarker for myocardial injury. When cardiac cells are damaged, cTn-I is rapidly released into the bloodstream, and its elevation is the gold standard for diagnosing acute myocardial infarction. In rat models, cTn-I quantification is essential for cardiovascular safety assessment and cardioprotective drug development.

Sandwich ELISA: Cardiac-Specific Detection with Dual-Antibody Capture

KTE101019 employs a two-site sandwich ELISA to quantitate cTn-I in samples. An antibody specific for cTn-I is pre-coated onto a microplate. Standards and samples are added, and any cTn-I present is captured by the immobilized antibody. After washing, a biotin-conjugated detection antibody specific for cTn-I is added, followed by Streptavidin-HRP. Substrate is added, and color develops in proportion to the amount of cTn-I bound[reference:36].

Rat-Specific, Cardiotoxicity-Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, and cell culture supernatants[reference:37]. No significant cross-reactivity or interference between Rat cTn-I and analogues was observed[reference:38].

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:39]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:40]
• Assay Duration: 3–5 hours[reference:41]
• Storage: 2–8°C unopened[reference:42]
• Shipping: Gel pack with blue ice[reference:43]

Complete Kit Components

The kit includes: Rat cTn-I microplate, Rat cTn-I standards, Rat cTn-I detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:44].

Who Needs This Kit?

• Cardiovascular researchers studying myocardial infarction and ischemia
• Toxicologists conducting regulatory cardiotoxicity assessments
• Pharmacologists developing cardioprotective drugs
• Drug safety scientists evaluating cardiac liability of novel compounds
• Translational researchers bridging rat models to human cardiac disease

🔗 Reference Link: https://www.abbkine.com/product/rat-cardiac-troponin-i-ctn-i-elisa-kit-kte101019/