KTE101023 | Rat Super Oxidase Dimutase (SOD) ELISA Kit — Quantify the Enzyme That Defends Against Superoxide Radicals

SOD: The First Line of Enzymatic Antioxidant Defense

Superoxide dismutase (SOD) is the enzyme that catalyzes the dismutation of the superoxide radical (O₂⁻) to hydrogen peroxide and molecular oxygen—one of the most critical antioxidant reactions in biology. SOD exists in three forms: cytosolic Cu/Zn-SOD (SOD1), mitochondrial Mn-SOD (SOD2), and extracellular SOD (SOD3). Together, they protect cells from the damaging effects of superoxide, a byproduct of mitochondrial respiration and a key driver of oxidative stress. In rat models, SOD activity or concentration is a core readout for oxidative stress status and antioxidant capacity.

Sandwich ELISA: Direct Quantification of a Key Antioxidant Enzyme

KTE101023 employs a sandwich ELISA format to quantitate SOD in samples. An antibody specific for SOD is pre-coated onto a microplate. Standards and samples are added, and any SOD present is captured by the immobilized antibody. After washing, a detection antibody is added, followed by HRP-conjugated secondary antibody. Substrate is added, and color develops in proportion to the amount of SOD bound.

Rat-Specific, Oxidative-Stress Research Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, tissue homogenates, and cell culture supernatants.

Specifications

• Assay Type: Sandwich ELISA (quantitative)
• Sample Types: Serum, Plasma, Tissue homogenates, Cell culture supernatants
• Assay Duration: 3–5 hours
• Storage: 2–8°C unopened
• Shipping: Gel pack with blue ice

Complete Kit Components

The kit includes: SOD microplate, SOD standards, SOD detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers.

Who Needs This Kit?

• Toxicologists assessing oxidative stress and antioxidant responses
• Cardiovascular researchers studying oxidative damage in heart disease
• Neurobiologists investigating neurodegeneration
• Hepatologists evaluating liver injury
• Aging researchers studying enzymatic antioxidant defenses

🔗 Reference Link: https://www.abbkine.com/product/rat-super-oxidase-dimutase-sod-elisa-kit-kte101023/