A21000 | HRP, Streptavidin — The Biotin-Binding Powerhouse That Amplifies Your Signal Without Amplifying Your Headaches

Biotin-Avidin: The Amplification System That Changed Immunodetection Forever

The biotin-streptavidin interaction is one of the strongest non-covalent bonds in biology—a relationship so stable it survives extreme pH, high temperatures, and denaturing conditions. When you pair this interaction with horseradish peroxidase (HRP), you get an amplification system that transforms faint signals into publication-ready bands and stains. Abbkine's HRP, Streptavidin (A21000) delivers this power with exceptional purity and consistency.

HRP-Conjugated Streptavidin: Why It Matters for Your Assays

Streptavidin binds to biotin with near-irreversible affinity (Kd ~10⁻¹⁴ M). When conjugated to HRP, it becomes a universal detection reagent for any biotin-labeled antibody, probe, or nucleic acid. Whether you're running a biotin-streptavidin ELISA, detecting biotinylated primary antibodies in Western blot, or visualizing biotinylated probes in IHC, A21000 gives you the sensitivity you need with minimal background.

Applications and Recommended Dilutions

A21000 is validated across three core applications with the following suggested starting dilutions [6†L4-L7]:

• Western Blot: 1:10,000–1:100,000
• IHC-P: 1:500–1:2,000
• ELISA: 1:5,000–1:100,000

Optimal dilutions should be determined experimentally, but these starting points will save you hours of titration.

Product Specifications

• Conjugate: HRP (horseradish peroxidase)
• Formulation: Liquid solution in PBS, pH 7.4, containing 1% BSA and 50% glycerol [6†L8-L9]
• Stability: Stable for one year at -20°C from date of shipment [6†L9-L10]
• Shipping: Gel pack with blue ice [6†L12]

Cited in 7 Publications — and Climbing

A21000 has already been cited in 7 publications, including a study on palmitic acid-triggered B7H3 palmitoylation promoting immune escape (Nature Communications, IF: 15.7) and research on V-ATPase E mediating Cry2Ab binding and toxicity in Helicoverpa armigera (Pesticide Biochemistry and Physiology, IF: 5) [6†L25-L33]. When your detection system needs to withstand peer review, this is the reagent you trust.

Who Needs This Reagent?

• Immunologists running biotin-streptavidin ELISA assays
• Cell biologists detecting biotinylated surface markers
• Pathologists performing IHC with biotinylated probes
• Molecular biologists using biotin-labeled nucleic acid probes

🔗 Reference Link: https://www.abbkine.com/product/hrp-streptavidin-a21000/