Human SFRP2 ELISA Kit — Application Scenarios for Cancer, Fibrosis, and Wnt Signaling Research

SFRP2: The Wnt Decoy You Can Finally Quantify

Secreted Frizzled-Related Protein 2 (SFRP2) is a soluble antagonist of Wnt ligands that modulates cell proliferation, differentiation, and tissue remodeling[reference:48]. Unlike the transmembrane Frizzled receptors that receive the Wnt signal, SFRP2 belongs to a family of soluble decoy regulators that lack the membrane-anchoring seven-transmembrane domain[reference:49]. Instead, SFRP2 is actively secreted into the extracellular space, where its cysteine-rich domain physically binds Wnt ligands — and can also interact with Frizzled receptors — effectively sequestering the signal away from the cell surface[reference:50]. Its dysregulation is implicated in cancer, fibrosis, and cardiovascular disease[reference:51].

The Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit (KTE60681) from Abbkine offers a rigorously validated solution for SFRP2 research[reference:52].

Liver Fibrosis Research: A Superior Biomarker

SFRP2 is upregulated in liver fibrosis (up to 8-fold vs. healthy controls) and correlates with disease severity[reference:53]. In a clinical validation, Abbkine tested the kit on 120 liver fibrosis patient samples, correlating SFRP2 levels with Ishak staging (r=0.85, p<0.001) — stronger than traditional markers like hyaluronic acid[reference:54]. Hepatology researchers use this kit to:

• Quantify SFRP2 in serum, plasma, and liver tissue lysates
• Monitor fibrosis progression and treatment response[reference:55]
• Investigate SFRP2 as a non-invasive biomarker for liver fibrosis staging
• Study the Wnt signaling pathway in hepatic stellate cell activation

The kit detects SFRP2 as low as 0.031 ng/mL in serum and 0.049 ng/mL in tissue lysates[reference:56], with a dynamic range of 0.031–6 ng/mL[reference:57].

Cancer Research: Tumor–Stroma Interactions

In pancreatic ductal adenocarcinoma (PDAC), SFRP2 is a marker of desmoplastic stroma[reference:58]. Generic kits have reported false positives in samples with high SFRP1, muddying correlations with patient survival[reference:59]. Oncology researchers use this kit to:

• Quantify SFRP2 in tumor lysates and patient plasma[reference:60]
• Study SFRP2-mediated Wnt pathway dysregulation in cancer[reference:61]
• Investigate SFRP2 as a prognostic biomarker and therapeutic target[reference:62]
• Monitor tumor–stroma interactions and metastatic progression[reference:63]

The kit's dual-epitope strategy — targeting the N-terminal cysteine-rich domain (residues 30–55, <5% homology to SFRP1/3) and the C-terminal netrin-like domain (residues 220–245) — slashes cross-reactivity to <1% with related SFRPs[reference:64].

Cardiovascular Disease and Cardiac Fibrosis

SFRP2 is one of the most upregulated secreted proteins in injured myocardium[reference:65]. Cardiovascular researchers use this kit to:

• Quantify SFRP2 in cardiac tissue lysates and patient serum[reference:66]
• Study Wnt pathway dysregulation in cardiac fibrosis
• Investigate SFRP2's role in pro-angiogenic paracrine signalling and cardiomyocyte survival[reference:67]

Why Choose This Kit for Your SFRP2 Research?

Kit Components at a Glance

• Human SFRP2 microplate (pre-coated with capture antibody)[reference:76]
• Human SFRP2 standard (7-point standard curve)[reference:77]
• Human SFRP2 detection antibody (biotin-conjugated)
• Streptavidin-HRP
• All buffers and reagents

🔬 Which research field does your work belong to?
Liver Fibrosis Research | Cancer Biology | Cardiovascular Research | Wnt Signaling | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: Human Secreted Frizzled-Related Protein 2 (SFRP2) ELISA Kit — KTE60681