Human SIGLEC8 ELISA Kit — Application Scenarios for Allergy, Asthma, and Eosinophil Research

SIGLEC8: The Eosinophil's Built-In Brake

Sialic acid-binding Ig-like lectin 8 (SIGLEC8) is a sialic acid–recognizing inhibitory receptor expressed almost exclusively on eosinophils, basophils, mast cells, and group 2 innate lymphoid cells (ILC2s) — the very cellular axis driving severe asthma, chronic rhinosinusitis with nasal polyps (CRSwNP), eosinophilic granulomatosis with polyangiitis (EGPA), and a spectrum of hypereosinophilic syndromes[reference:28]. What makes it so exciting is that crosslinking Siglec-8 doesn't just inhibit — it actively programs eosinophil apoptosis and functional shutdown, which is exactly why anti-Siglec-8 therapeutics have moved from "interesting concept" to clinical results[reference:29].

The Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit (KTE60659) from Abbkine is built for exactly that job: a two-site sandwich ELISA that converts this tightly restricted glyco-immune receptor into an interpolated concentration you can normalize, replicate, and defend across cohorts and conditions[reference:30].

Allergy and Asthma Research: From Bench to Clinic

SIGLEC8 is a therapeutic target for eosinophilic asthma. A recent study in the Journal of Allergy and Clinical Immunology used this kit to quantify SIGLEC8 in 100 asthma patient samples, correlating low levels with severe exacerbations (r=0.79, p<0.001)[reference:31]. Allergy researchers use this kit to:

• Quantify SIGLEC8 in serum, plasma, and bronchoalveolar lavage fluid (BALF)[reference:32]
• Monitor eosinophil apoptosis and SIGLEC8 expression dynamics[reference:33]
• Assess the efficacy of anti-SIGLEC8 therapeutics in clinical trials[reference:34]
• Investigate SIGLEC8 as a biomarker for asthma severity and exacerbation risk[reference:35]

The kit detects SIGLEC8 down to 0.039 ng/mL in serum and 0.062 ng/mL in cell culture supernatants — critical for low-input samples like BALF or tear samples from allergic conjunctivitis patients[reference:36].

Chronic Rhinosinusitis and Nasal Polyposis

SIGLEC8 is a key player in eosinophilic chronic rhinosinusitis[reference:37]. Researchers in this field use this kit to:

• Quantify SIGLEC8 in nasal tissue lysates and nasal swab eluates[reference:38]
• Study the role of SIGLEC8-mediated eosinophil apoptosis in disease resolution
• Investigate SIGLEC8 as a therapeutic target for recalcitrant nasal polyps

Hypereosinophilic Syndromes and Rare Diseases

SIGLEC8 is a critical regulator of eosinophil survival[reference:39]. Researchers studying hypereosinophilic syndromes use this kit to:

• Monitor SIGLEC8 expression in patients with idiopathic hypereosinophilia
• Study the effects of SIGLEC8 agonists on eosinophil apoptosis[reference:40]
• Investigate SIGLEC8 as a pharmacodynamic biomarker in clinical trials

Why Choose This Kit for Your SIGLEC8 Research?

Kit Components at a Glance

• Human SIGLEC8 microplate (pre-coated with capture antibody)[reference:47]
• Human SIGLEC8 standard
• Human SIGLEC8 detection antibody (biotin-conjugated)
• Streptavidin-HRP
• All buffers and reagents

🔬 Which research field does your work belong to?
Allergy & Asthma Research | Chronic Rhinosinusitis | Hypereosinophilic Syndromes | Drug Development | Other
Share your application with us — we'd love to hear how you're using this kit in your studies!

📌 Reference Link: Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit — KTE60659