KTE100827 | Rat Nuclear factor-kappa B (NF-kB) ELISA Kit — Quantify the Master Transcription Factor of Inflammation and Immunity

NF-kB: The Conductor of the Inflammatory Symphony

Nuclear factor-kappa B (NF-kB) is arguably the most important transcription factor in immunology—a family of proteins that controls the expression of genes involved in inflammation, immunity, cell proliferation, and survival. NF-kB activation is triggered by cytokines (TNF-α, IL-1), pathogens, and stress signals, making it a central player in everything from infectious disease to cancer. In rat models, NF-kB quantification is essential for studying inflammatory disease, drug response, and immune regulation.

Sandwich ELISA: Direct Quantification of a Nuclear Transcription Factor

KTE100827 employs a two-site sandwich ELISA to quantitate NFKB1 (p50) in samples. An antibody specific for NFKB1 is pre-coated onto a microplate. Standards and samples are added, and any NFKB1 present is captured by the immobilized antibody. After washing, a biotin-conjugated detection antibody specific for NFKB1 is added, followed by Streptavidin-HRP. Substrate is added, and color develops in proportion to the amount of NFKB1 bound[reference:17].

Rat-Specific, Inflammation-Research Ready

With reactivity validated exclusively for Rat, this kit is specifically optimized for rat serum, plasma, tissue lysates, and cell culture supernatants. No significant cross-reactivity or interference between Rat NFKB1 and analogues was observed[reference:18].

Specifications

• Assay Type: Sandwich ELISA (quantitative)[reference:19]
• Sample Types: Cell culture supernatants, Plasma, Serum, Other biological fluids[reference:20]
• Assay Duration: 3–5 hours[reference:21]
• Storage: 2–8°C unopened[reference:22]
• Shipping: Gel pack with blue ice[reference:23]

Complete Kit Components

The kit includes: Rat NF-kB microplate, Rat NF-kB standards, Rat NF-kB detect antibody, Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers[reference:24].

Who Needs This Kit?

• Immunologists studying inflammatory signaling pathways
• Cancer researchers investigating NF-kB in tumorigenesis
• Cardiovascular researchers studying atherosclerosis and inflammation
• Neurobiologists investigating neuroinflammation
• Drug developers screening NF-kB inhibitors

🔗 Reference Link: https://www.abbkine.com/product/rat-nuclear-factor-kappa-b-nf-kb-elisa-kit-kte100827/